Abstract
Fluxes of ions acros the plasma membrane are associated with the signal transduction of different intra- and extracellular stimuli such as the plant hormones auxin, abscisic acid and gibberellins. In these plant hormone signal transduction processes ion channels are involved. To be able to understand the significance and role of ion channels in plant hormone signal transduction an inventory and characterization of the plasma membrane ion channels in the plant cell of interest should be carried out.
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References
An G (1985) High efficiency transformation of cultured tobacco cells. Plant Physiol 79: 568–570
Boot CJM, Van Duijn B, Mennes AM, Libbenga KR (1996) Regulation of a class of auxin-induced genes in cell suspension cultures from Nicotiana tabacum. In: Smith AR, Berry AW, Harpham NVJ, Moshkov IE, Novikova GV, Kulaeva ON, Hall MA (eds) Plant hormone signal perception and transduction. Kluwer Academic, Dordrecht, pp 41–48
Dangle JL, Hauffe KD, Lipphardt S, Hahlbrock K, Scheel D (1987) Parsley protoplasts retain differential responsiveness to u.v light and fungal elicitor. EMBO J 6: 2551–2556
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127
Thomine S, Zimmermann S, Van Duijn B, Barbier-Brygoo H, Guern J (1994) Calcium channel antagonists induce direct inhibition of the outward rectifying potassium channel in tobacco protoplasts. FEBS Lett 340: 45–50
Van der Zaal EJ, Mennes AM, Libbenga KR (1987) Auxin-induced rapid changes in translatable mRNA’s in tobacco tell suspensions. Planta 172: 514–519
Van Duijn B, Ypey DL, Libbenga KR (1993) Whole cell K+ currents across the plasma membrane of tobacco protoplasts from cell suspension cultures. Plant Physiol 101: 81–88
Van Duijn B (1993) Hodgkin-Huxley analysis of whole cell outward rectifying K+ currents in protoplasts from tobacco cell suspension cultures. J Membrane Biol 132: 77–85
Van Duijn B, Heimovaara-Dijkstra S (1994) Intracellular microelectrode membrane potential measurements in tobacco cell suspension protoplasts and barley aleurone protoplasts: interpretation and artifacts. Biochim Biophys Acta 1193: 77–84
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© 1998 Springer-Verlag Berlin Heidelberg
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van Duijn, B. (1998). Measurement of Whole Cell Potassium Currents in Protoplasts from Tobacco Cell Suspensions. In: Van Duijn, B., Wiltink, A. (eds) Signal Transduction — Single Cell Techniques. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80368-0_20
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DOI: https://doi.org/10.1007/978-3-642-80368-0_20
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-48976-1
Online ISBN: 978-3-642-80368-0
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