Abstract
During development, gene expression is tightly regulated in time and space. The specific expression pattern of a particular gene results from the combination of several parameters, which include:
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The presence in its promoter of sequences binding specific trans-acting proteins
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The expression of these trans-acting proteins which, when bound to their target sequence, determine the activation or repression of transcription
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An adequate chromatin conformation that, in its open conformation, allows binding of transcription factors to their target sequences.
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Chung JH, Whiteley M, Felsenfeld G (1993) A 5’ element of the chicken (3-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. Cell 74: 505–514
Escalante-Alcalde D, Recillas-Targa F. Hernandez-Garcia D, Castro-Obregön S, Terao M, Garattini E, Covarrubias L (1996) Retinoic acid and cis-methylation regulatory elements control the mouse tissue non-specific alkaline phosphatase gene expression. Mech Dev 57: 21–32
Festenstein R, Tolain I N, Corbella P, Mamalaki C, Parrington J, Fox M, Miliou A, Jones M, Kioussis D (1996) Locus control region function and heterochromatininduced position effect variegation. Science 271: 1123–1125
Fiering S, Kim CG, Epner E, Groudine M (1993) An “in-out” strategy using gene targeting and FLP recombinase for the functional dissection of complex DNA regulatory elements: Analysis of the (3-globin locus control region. Proc Natl Acad Sci USA 90: 8469–8473
Fiering S, Epner E, Robinson K, Zhuang Y, Telling A, Hu M, Martin DJK, Enver T, Levy TJ, Groudine M (1995) Targeted deletion of 5’ HS2 of the murine 13-globin LCR reveals that it is not essential for proper regulation of the (3-globin locus. Genes Dev 9: 2203–2213
Gordon J (1993) Production of transgenic mice. Methods Enzymol 225: 747–771
Hogan B, Beddington R, Costantini F, Lacy E (1994) Manipulating the mouse embryo: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Mann JR, McMahon AP (1993) Factors influencing frequency production of trans-genic mice. Methods Enzymol 225: 771–781
Ramirez-Solis R, Davis AC, Bradley A (1993) Gene targeting in embryonic stem cells. Methods Enzymol 225: 855–878
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Schedl A, Larin Z, Montoliu L, Thies E, Kelsey G, Lehrach H, Schutz G (1993) A method for the generation of YAC transgenic mice by pronuclear microinjection. Nucleic Acids Res 21: 4783–4787
Studer M, Terao M, Gianni M, Garttini E (1991) Characterization of a second promoter for the mouse liver/bone/kidney-type alkaline phosphatase gene: cell and tissue specific expression. Biochem Biophys Res Commun 179: 1352–1360
Terao M, Studer M, Gianni M, Garattini E (1990) Isolation and characterization of the mouse liver/bone/kidney-type alkaline phosphatase gene. Biochem J 268: 641–648
Wolfe AP (1994) Insulating chromatin. Curr Biol 4: 85–87
Wuenschell CW, Mori M, Anderson DJ (1990) Analysis of SCG10 gene expression in transgenic mice reveals that neural specificity is achieved through selective derepression. Neuron 4: 595–602
Zambrowicz BP, Zimmermann JW, Harendza CJ, Simpson EM, Page DC, Brinster RL, Palmiter RD (1994) Expression of a mouse Zfy-1/lacZ transgene in the somatic cells of embryonic gonad and germ cells of the adult testis. Development 120: 1549–1559
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© 1998 Springer-Verlag Berlin Heidelberg
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Escalante-Alcalde, D., Covarrubias, L. (1998). Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements. In: Cid-Arregui, A., García-Carrancá, A. (eds) Microinjection and Transgenesis. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80343-7_22
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DOI: https://doi.org/10.1007/978-3-642-80343-7_22
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