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Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements

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Microinjection and Transgenesis

Part of the book series: Springer Lab Manual ((SLM))

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Abstract

During development, gene expression is tightly regulated in time and space. The specific expression pattern of a particular gene results from the combination of several parameters, which include:

  • The presence in its promoter of sequences binding specific trans-acting proteins

  • The expression of these trans-acting proteins which, when bound to their target sequence, determine the activation or repression of transcription

  • An adequate chromatin conformation that, in its open conformation, allows binding of transcription factors to their target sequences.

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© 1998 Springer-Verlag Berlin Heidelberg

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Escalante-Alcalde, D., Covarrubias, L. (1998). Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements. In: Cid-Arregui, A., García-Carrancá, A. (eds) Microinjection and Transgenesis. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80343-7_22

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  • DOI: https://doi.org/10.1007/978-3-642-80343-7_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-61895-9

  • Online ISBN: 978-3-642-80343-7

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