Advertisement

Properties of an STR multiplex marker system suitable for paternity and forensic determinations

  • J. Neuweiler
  • L. Perlee
  • J. Venturini
  • I. Balazs
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 6)

Abstract

Simple sequence repeat polymorphisms composed of tandem di-,tri- or tetranucleotide repeats (STR) are highly abundant in the human genome (Williamson et al. 1990) and are particularly suitable for detection by PCR amplification. These type of loci have been used for population genetic studies, determination of paternity or forensic analysis (Edwards et al. 1991, Kimpton et al. 1993). Analysis of these markers has been performed either by individual amplification of single loci or by the simultaneous amplification of several loci (i.e. multiplexing). Alleles can be detected by gel electrophoresis followed by radioactive labeling, silver staining or fluorescence detection.

Keywords

Short Tandem Repeat Locus Forensic Analysis Tetranucleotide Repeat Random Individual Combine Power 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49:746–756PubMedGoogle Scholar
  2. Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M (1993) Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR Methods and Applications 3:13–22PubMedGoogle Scholar
  3. Perlee L, Neuweiler J, Balazs I (1995) Analysis of sequence variations in the alleles from three STR loci, this proceeding.Google Scholar
  4. Williams R, Bowcock A, Kidd K, Pearson P, Schmidtke J, Chan HS et al. (1990) Report of the committee and catalogues of cloned and mapped genes and DNA polymorphisms. Cytogenet Cell Genet 55:457–778CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • J. Neuweiler
    • 1
  • L. Perlee
    • 1
  • J. Venturini
    • 1
  • I. Balazs
    • 1
  1. 1.Lifecodes Corp.StamfordUSA

Personalised recommendations