Automated Analysis of 5 STR Loci: Allele Frequencies and Family Studies in the German Population

  • J. Reinhold
  • J. Arnold
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 6)

Abstract

Analysis of polymorphic DNA loci by the polymerase chain reaction (PCR) technique is a very powerful tool not only in the field of criminal investigation but also in paternity testing. Compared to VNTR systems typed by Southern Blot hybridisation, STR elements offer considerable advantages concerning sensitivity, precision and reproducibility of fragment size estimates and biomathematical evaluation.

Keywords

Electrophoresis Polyacrylamide 

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References

  1. Kimpton C. P., Gill P., Walton A., Urquhart A., Millican E. S. and Adams M. (1993). PCR Methods and Applications. 3: 13–22PubMedGoogle Scholar
  2. Miller S. A., Dykes D. D. and Polesky H. F. (1988). A salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 16: 1215PubMedCrossRefGoogle Scholar
  3. Schmitter H., Sonntag M.-L. (1995). Proposal for general rules for the denominations of alleles of STR-loci based on the electrophoretic mobilities of their fragments. Klin. Lab. 41: 173–176Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • J. Reinhold
    • 1
  • J. Arnold
    • 1
  1. 1.Institut für LabormedizinWürzburgGermany

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