Automation of In Situ DNA Sample Preparation for PCR Using the FTA™ DNA Collection System and the Rosys Laboratory Workstation
The development of dried blood stain technology has greatly facilitated the collection, transport, and isolation of DNA from blood samples. Blood stain cards are currently being used in clinical screening programs for Cystic Fibrosis (Audrezet and Costes, 1993; Raskin, 1992) and Human Immunodeficiency Virus (HIV) (Nyambi, 1994; Cassol, 1991). Within the forensic community, blood stain cards are being used to collect DNA samples from convicted felons and/or sex offenders, as military reference samples (Williams et al., 1994), and for parentage testing. Current blood stain technology, which is based on the use of filter paper cards, has provided a simple system for the collection, transport, and storage of whole blood DNA samples. However, the DNA must be extracted from the blood stain card prior to amplification and analysis.
KeywordsMigration Magnesium Phenol Agarose Bromide
Unable to display preview. Download preview PDF.
- 2.Cassol, S. (1991). Use of Dried Blood Spot Specimens in the Detection of Human Immunodeficiency Virus Type I by the Polymerase Chain Reaction. J. Clinical Microbiology. 29: 661–667Google Scholar
- 4.Nyambi, P., Fransen, K., De Beenhouwer, H., Ephantus, N., Temmerman, M., Ndinya-Achola, J., Piot, P., and van der Groen, G. (1994). Detection of Human Immunodeficiency Virus type 1 (HIV-1) in Heel Prick Blood on Filter Paper from Children Born to HIV-1-Seropositive Mothers. J. of Clin. Microbiol. Nov. p. 2858–2860.Google Scholar
- 5.Williams, P., Marino, M., Del Rio, S., Turner, K., Steighner, B., Burgoyne, L., and Turner, J. (1994). Evaluation of a Novel Matrix for the Analysis of DNA and its Application to Automated DNA sample processing. Proceedings from Fifth International Symposium on Human Identification, Promega Corp.Google Scholar
- 8.Gregory, C., Myal, Y., and Shiu, R. (1995). Rapid Genotyping of Transgenic Mice Using Dried Blood Spots from Guthrie Cards for PCR Analysis. Biotechniques Vol. 18, No.5 759–760.Google Scholar
- 9.Harvey, M., King, T., Burghoff, R. (1995). Impregnated 903 Blood Collection Paper: A Tool for DNA Prepared From Dried Blood Spots for PCR Amplification. Presented at: American Association of Clinical Chemistry, 1995, Anehiem, CA.Google Scholar