Abstract
The use of directly labeled alkaline phosphatase oligonucleotides (AP-probes) and chemiluminescent substrates for the analysis of VNTR loci has proven to be a simple, rapid and convenient format for DNA typing(Baum, 1990, Neuweiler 1992, Balazs 1994 and Baird 1994). The advent of new and more sensitive chemiluminescent substrates for alkaline phosphatase has provided the identity laboratory with even more powerful chemiluminescent probes for DNA typing. This paper discusses the development of a protocol for the use of a new, highly-sensitive alkaline phosphatase substrate, CDP-Star™ (Tropix Inc., Bedford, MA) with a number of AP-probes and with either charged or neutral membranes.
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References
Baird M, Galbreath L, Cunningham R, Lastella J, Balazs I (1994) Use of chemiluminescent labeled probes for forensic and paternity determinations. In: Bar W and Rossi U (eds) Advances in Forensic Haemogenetics, vol 5. Springer-Verlag, Berlin Heidelberg, p 130.
Balazs I, Neuweiler J, Kowalski K, Victor J (1994) Streamlining VNTR analysis. A fast procedure for non-isotopic DNA profiling. In: Bar W and Rossi U (eds) Advances in Forensic Haemogenetics, vol 5. Springer-Verlag, Berlin Heidelberg, p 112.
Baum HJ, Fitz-Charles H, McKee R (1990) A non-isotopic DNA detection system with the sensitivity of P32: Applications for paternity and forensic identifications. In: Polesky HF and Mayr WR (eds) Advances in Forensic Haemogenetics, vol 3. Springer-Verlag, Berlin Heidelberg, p 37.
Neuweiler J, Venturini J, Balazs I, Komher S, Hintz D, Victor, J (1992) The use of a chemiluminescent detection system for paternity and forensic testing. Verification of the reliability of the oligonucleotide-probes used for genetic analysis. In: Rittner C and Schneider PM (eds) Advances in Forensic Haemogenetics, vol 4. Springer-Verlag, Berlin Heidelberg, p 132.
Price DC Chemiluminescent substrates for detection of restriction fragment length polymorphism. Journal Forensic Science Society, in press.
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© 1996 Springer-Verlag Berlin Heidelberg
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Leary, S.L., Victor, J., Balazs, I. (1996). Use of CDP-STAR in a fast and highly sensitive chemiluminescent detection procedure for VNTR loci with neutral and charged membranes. In: Carracedo, A., Brinkmann, B., Bär, W. (eds) 16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995. Advances in Forensic Haemogenetics, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80029-0_101
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DOI: https://doi.org/10.1007/978-3-642-80029-0_101
Publisher Name: Springer, Berlin, Heidelberg
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