Skip to main content
SpringerLink
Log in

Achievements in the Genetics of Nitrogen Fixation in Hungary

  • Conference paper
Book cover Azospirillum VI and Related Microorganisms

Part of the book series: NATO ASI Series ((ASIG,volume 37))

  • 430 Accesses

Abstract

The research development of microbial and molecular genetics is followed by the results on the genetics of Rhizobium meliloti and its phages. Two temperate and one virulent Rhizobium phage was characterized. By the established, original phage genetic system genetic and physical maps were constructed, genes identified as well as molecular mechanisms in temperate regulation, phage-bacterium interactions clarified. Transduction was worked out for mapping and for transposon suicide transfer into defective lysogenic recipients. Transformation and transfection was carried out with R. meliloti. The bacterial genome was mapped by transduction and conjugation. The nod and nif genes were localized, characterized, the genetic communication between Rhizobium meliloti and alfalfa revealed with molecular genetic techniques. Plant host specific chemotaxis of Rhizobia was demonstrated and genes involved identified. For practical application and strain improvement a competition test was elaborated including the identification of reisolated symbionts by phage superinfection.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Balassa R (1956) Durch Desoxyribonukleinsäuren induzierte Veränderungen an Rhizobien. Naturwissenschaften 43: 133–134

    Article  Google Scholar 

  • Balassa R (1960) Transformation of a strain of Rhizobium lupini. Nature 188: 246–247

    Article  PubMed  CAS  Google Scholar 

  • Balassa R, Gabor M (1964) Transformation of Streptomycin markers in rough strains of Rhizobium lupini. Acta Microbiologica Acad. Sci. Hu. 11: 329–339

    CAS  Google Scholar 

  • Bánfalvi Z, Randhava GS, Kondorosi E, Kiss A, Kondorosi A (1983) Construction and characterization of R-prime plasmids carrying symbiotic genes of Rhizobium. Mol. Gen. Genet. 189: 129–135

    Article  Google Scholar 

  • Bánfalvi Z, Sakanyan V, Koncz Cs, Kiss A, Dusha I, Kondorosi A (1981) Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of Rhizobium meliloti. Mol. Gen. Genet. 184: 318–325

    PubMed  Google Scholar 

  • Barabäs I, Sík T (1979) Phenotypic reversion of nitrogenase in pleiotropic mutants of Rhizobium meliloti. Can. J. Microbiol. 25: 298–301

    Article  PubMed  Google Scholar 

  • Dallmann G, Papp P, Orosz L (1987) Related repressor specificity of unrelated phages. Nature 330: 298–401

    Article  Google Scholar 

  • Dallmann G, Orosz L, Sain B (1979) Restriction mapping of DNA of temperate Rhizobium meliloti phage 16-3: Comparison of genetic and physical maps indicates a long genetically silent chromosomal arm. Mol. Gen. Genet. 176: 439–448

    Article  PubMed  CAS  Google Scholar 

  • Dusha I, Bakos Á, Kondorosi A, de Bruijn FJ, Schell J (1989) The Rhizobium meliloti early nodulation genes (nodABC) are nitrogen-regulated: Isolation of a mutant strain with efficient nodulation capacity on alfalfa in the presence of ammonium. Mol. Gen. Genet. 219: 89–96

    Article  CAS  Google Scholar 

  • Dusha I, Kondorosi A (1993) Genes at different regulatory levels are required for the ammonia control of nodulation in Rhizobium meliloti. Mol. Gen. Genet. 240: 435–444

    PubMed  CAS  Google Scholar 

  • Dusha I, Kovalenko S, Bänfalvi Z, Kondorosi A (1987) Rhizobium meliloti insertion element ISRm2 and its use for identification of the fixX gene. J. Bacterid. 169: 1403–1409

    CAS  Google Scholar 

  • Györgypál Z, Narayan Iyer, Kondorosi A (1988) Three regulatory nodD alleles of diverged flavonoid-specificity are involved in host-dependent nodulation by Rhizobium meliloti. Mol. Gen. Genet. 212: 85–92

    Article  Google Scholar 

  • Kárpáti E, Ponyi T, Sik T (1989) Host-specific Chemotaxis of Rhizobium meliloti induced by alfalfa. Bull. Univ. Agr. Sci. Gödöllö 15–19

    Google Scholar 

  • Kiss GB, Dobo K, Dusha I, Breznoivits Ä, Orosz L, Vincze E, Kondorosi A (1980) Isolation and characterization of an R-prime plasmid in Rhizobium meliloti. J. Bacteriol. 141: 121–128

    PubMed  CAS  Google Scholar 

  • Kiss GB, Kálmán Z (1982) Transformation of Rhizobium meliloti 41 with plasmid DNA. J. Bacteriol. 150: 465–470

    PubMed  CAS  Google Scholar 

  • Kondorosi A, Barabás I, Sváb Z, Orosz L, Sík T, Hotchkiss RD (1973) Evidence for common genetic determinants of nitrogenase and nitrate reductase in Rhizobium meliloti. Nature, New Biol. 246: 153–154

    CAS  Google Scholar 

  • Kondorosi A, Kiss GB, Forrai T, Vincze E, Bänfalvi Z (1977) Circular linkage map of Rhizobium meliloti chromosome. Nature 268: 525–527

    Article  Google Scholar 

  • Kondorosi A, Orosz L, Sväb Z, Sik T (1974) Genetic studies on Rhizobiophage 16-3. II. Helper-induced Transfection. Mol. Gen. Genet. 132: 153–163

    PubMed  CAS  Google Scholar 

  • Kondorosi E, Bánfalvi Z, Kondorosi A (1984) Physical and genetic analysis of a symbiotic region of Rhizobium meliloti: Identification of nodulation genes. Mol. Gen. Genet. 193: 445–452

    Article  CAS  Google Scholar 

  • Mink M, Orosz L, Sík T (1982) Specialized and generalized transducing Rhizobiophage 16-3 and 11 are closely related. FEMS Microbiol. Lett. 13: 383–387

    Article  CAS  Google Scholar 

  • Olasz F, Dorgai L, Páy A, Orosz L (1983) Recombination Deficient Mutants of Rhizobium meliloti 41. Mol. Gen. Genet. 191: 393–396

    Article  PubMed  CAS  Google Scholar 

  • Orosz L (1980) Methods for analysis of the C cistron of temperate phage 16-3 of Rhizobium meliloti. Genetics 94: 265–276

    PubMed  CAS  Google Scholar 

  • Orosz L, Páy A, Dallmann G (1980) Heterozygosis of phage 16-3 of Rhizobium meliloti. Moderate level of mismatch repair or gene conversion. Mol. Gen. Genet. 179: 163–167

    Article  PubMed  CAS  Google Scholar 

  • Orosz L, Rostás K, Hotchkiss RD (1980) A comparison of two-point, three-point and deletion mapping in the C cistron of Rhizobiophage 16-3, with an explanation for the recombination pattern. Genetics 94: 249–263

    PubMed  CAS  Google Scholar 

  • Orosz L, Sváb Z, Kondorosi A, Sík T (1973) Genetic studies on Rhizobiophage 16-3. I. Genes and functions on the chromosome. Mol. Gen. Genet. 125: 341–350

    PubMed  CAS  Google Scholar 

  • Papp I,Dorgai L, Papp P, Jónás E, Olasz F, Orosz L (1993) The bacterial attachment site of temperate Rhizobium phage 16-3 overlaps the 3′ end of a putative proline tRNA gene. Mol. Gen. Genet. 240: 258–264

    Google Scholar 

  • Putnoki P, Petrovics G, Kereszt A, Grosskopf E, Dang thi Cam Ha, Bánfalvi Z, Kondorosi A (1990) Rhizobium meliloti lipopolysaccharide and exopolysaccharid can have the same function in the plant-bacterium interaction. J. Bacterid. 172: 5450–5458

    Google Scholar 

  • Sík T, Barabás I (1977) The correlation of nitrate reductase and nitrogenase in Rhizobium symbiosis. In: Recent Developments in nitrogen fixation (Eds. Newton W, Postgate JR, Rodríguez-Barrueco C) Academic Press Inc. pp. 365–373

    Google Scholar 

  • Sík T, Horváth J, Chatterjee S (1980) Generalized transduction in Rhizobium meliloti. Mol. Gen. Genet. 178: 511–516

    Article  PubMed  Google Scholar 

  • Sík T, Kondorosi A, Barabás I, Sváb Z (1976) Nitrate reductase and effectiveness in Rhizobium. In: Proceedings of the 1st International Symposium on nitrogen fixation (Eds. Newton WE, Nyman CJ) Washington State University Press Vol. 2 pp. 374–382

    Google Scholar 

  • Sík T, Orosz L (1971) Chemistry and genetics of Rrizobium meliloti phage 16-3. Plant and Soil Sp.Vol.: 57–62

    Google Scholar 

  • Sváb Z, Kondorosi A, Orosz L (1978) Specialized transduction of cysteine marker by Rhizobium meliloti phage 16-3. J. Gen. Microbiol. 106: 321–327

    Google Scholar 

  • Szende K, Órdogh F (1960) Die Lysogenie von Rhizobium meliloti. Naturwissenschaften 47: 404–405

    Article  Google Scholar 

  • Szende K, Sík T, Órdogh F, Gyorffy B (1961) Transfer of immunity by nucleic acids of a lysogenie Rhizobium strain. Biochim. Biophys. Acta 47: 215–217

    Article  PubMed  CAS  Google Scholar 

  • Tórók I, Kondorosi A (1981) Sequence of the Rhizobium meliloti nitrogen reductase (nif H) gene. Nucl. Acids Res. 9: 5711–5723

    Article  PubMed  Google Scholar 

  • Tórók I, Kondorosi E, Stepkowski T, Pósfai J, Kondorosi A (1984) Nucleotide sequence of Rhizobium meliloti nodulation genes. Nucleic Acids Res. 12: 9509–9524

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, University of Agricultural Sciences, H-2103, Gödöllő, Hungary

    Tibor Sík

Authors
  1. Tibor Sík
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute of Biophysics, University of Hannover, Herrenhäuser Straße 2, D-30419, Hannover, Germany

    Istvàn Fendrik

  2. Department of Food Technology, Environmental and Microbiological Sciences, University of Molise, Via Tiberio 21, I-86100, Campobasso, Italy

    Maddalena del Gallo

  3. F. A. Janssens Laboratory of Genetics Department of Applied Plant Sciences, K. U. Leuven, Willem de Croylaan 42, B-3001, Heverlee, Belgium

    Jos Vanderleyden

  4. Département des Biotechnologies, Institut Pasteur, Unité de Physiologie Cellulaire, CNRS URA 1300, F-75724, Paris Cedex 15, France

    Miklos de Zamaroczy

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Sík, T. (1995). Achievements in the Genetics of Nitrogen Fixation in Hungary. In: Fendrik, I., del Gallo, M., Vanderleyden, J., de Zamaroczy, M. (eds) Azospirillum VI and Related Microorganisms. NATO ASI Series, vol 37. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79906-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-79906-8_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79908-2

  • Online ISBN: 978-3-642-79906-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation