Chitinase Expression in Strawberry Root Colonized by Azospirillum Brasilense and V. A. Mycorrhiza

  • Carlos H. Bellone
  • Silvia C. de Bellone
  • Raul O. Pedraza
Part of the NATO ASI Series book series (volume 37)


Spores of Glomus intraradix and Azospirillum brasilense were inoculated on 5 varieties of Strawberry. In all case, both microorganisms develop chitinase expression on plants, finding that the highest values of this enzyme are produced when the inoculation is altogether with both microorganisms. Associated to this process it was found increments in the number of bacteria and nitrogenase activity with the dual inoculation.


Mycorrhizal Fungus Cane Root Chitinase Activity Strawberry Plant Azospirillum Brasilense 
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  1. Benhamou N Chamberland H Panze E J (1990) Implication of pectic components in cell surface interaction between tomato root cells and Fusarium oxysporum spradicis Iycopersisi. Plant physiol 92: 995–1003.PubMedCrossRefGoogle Scholar
  2. Bellone C H (1983) Incrementos de la actividad celulolitica en tejidos no diferenciados de cana de azúcar (Saccharum spp L) cultivados “in vitro” e inoculados con dos especies de Azospirillum. Revista Agronómica del Noroeste Argentino vol XX (1–4): 5–15.Google Scholar
  3. Bellone C H Bellone S C de (1993) Nitrogenase cellulase and pectinase activity in sugar cane roots inoculated together with mycorrhiza and nitrogen fixing bacteria. VI international symposium of the nitrogen fixing microorganisms in leguminous plant. Egypt.Google Scholar
  4. Bonfante-Fasolo P Grippioli R (1982) Cytochemical and biochemical observation on the cell wall of the spore of Glomus epigaeous. Protoplasma 123: 140–151.CrossRefGoogle Scholar
  5. Boiler T Gehri A Manch F Vogelli V (1983) Chitinase in bean leaves induction by ethylene purification properties and possible functions. Planta 157: 22–31.CrossRefGoogle Scholar
  6. Bradford M M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 72: 248–254.PubMedCrossRefGoogle Scholar
  7. Dóbereiner J De Polli H (1980) Nitrogen fixation in rhizocoenosses. International symposium on roots soil system. Londrina. Brasil.Google Scholar
  8. Bowles D J (1990) Defense-related proteins in higher plants. Annu Rev Biochem 59: 873–907.PubMedCrossRefGoogle Scholar
  9. Broekaert W F Peumans W J (1988) Pectic polysaccharides elicit chitinase accumulations in tobacco. Physiol Plant 74: 740–44.CrossRefGoogle Scholar
  10. Genderman J W (1968) Vesicular-Arbuscular mycorrhiza and plant growth. Anual Review of Phytopathology 6: 397–418CrossRefGoogle Scholar
  11. Giovanneti M Mosse B (1980) An evaluation of techniques for measuring vesicular- arbuscular mycorrhizal infection in roots. J New phytol 84: 489–500.CrossRefGoogle Scholar
  12. Metraux J P Boiler T (1986) Local and systemic induction of chitinase in cucumber plants in response to viral bacterial and fungal infections. Physiol Plant Phathol 28: 161–69.CrossRefGoogle Scholar
  13. Murashige T Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant physiol 15: 473–497.CrossRefGoogle Scholar
  14. Newman EI Reddell P (1987) The distribution of mycorrhizas among families of vascular plants. New phytol 106: 745–751.CrossRefGoogle Scholar
  15. Okon Y (1985) Azospirillum as a potential inoculated for agriculture. Trends Biotechnol 3: 223–228CrossRefGoogle Scholar
  16. Okon Y, Labandera-Gonzáles C A (1994) Agronomic application of Azospirillum: an evaluation of 20 years world wide field inoculation. Soil Biol. Biochem (in press).Google Scholar
  17. Roby D Gadelle A Toppan A (1987) Chitin oligosaccharides as elictiors of chitinase activity in melon plants. Biochem. Biophys Rev Commun 143: 885–92.CrossRefGoogle Scholar
  18. Schulumbaum A Manch F Vogeli V Boiler T (1986) Plant chitinases are potent inhibitors of fungal growth. Nature 324: 365–367.CrossRefGoogle Scholar
  19. Spanu P Boiler T Ludwig A Wiemken A Faccio A (1989) Chitinase in roots of mycorrhizal A Ilium porrum regulation and localization. Planta 177: 447–55.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Carlos H. Bellone
    • 1
  • Silvia C. de Bellone
    • 1
  • Raul O. Pedraza
    • 1
  1. 1.Departament of EcologyUniversity of Tucuman5 N de TucumanArgentina

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