Skip to main content

Biosensors in pharmacology and toxicology in vitro

  • Conference paper
Modulation of Cellular Responses in Toxicity

Part of the book series: NATO ASI Series ((ASIH,volume 93))

  • 124 Accesses

Abstract

The need of disposing of analytical information in real time in many fields of bioanalytical and bioindustries explains the great attention devoted to biosensor development. A biosensor is usually defined as resulting from the combination of a sensitive biological element capable of molecular recognition and a transductor (electrode, optical detector…) which gives a meaningful mostly electrical signal. During the last decade, major development has concerned electrode tipped with enzymes, antibodies or other reagents that interact chemically with analytes -the substances being analysed. Such biosensors are expected to have numerous applications in the field of medicine and biology (Brennan and Krull,1992).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliography

  • Bagley, D.M., Bruner, L.H., de Silva, O., Cottin, M., O’Brien, K.A.F., Uttley, M., Walker, A.P. (1991). An evaluation of five potential in vitro alternatives to the in vivo rabbit eye irritation test. Toxic. In Vitro, 5, 277–284.

    Article  Google Scholar 

  • Baxter, G.T., Miller, D.L., Kuo, RC., Wada, H.G., Owicki, J.C. (1992). PKCs is involved in granulocyte-macrophage colony-stimulating factor signal transduction: evidence from microphysiometry and antisense oligonucleotide experiments. Biochemistry, 31, 10950–10954.

    Article  PubMed  CAS  Google Scholar 

  • Brennan, J.D., Krull, U.J. (1992). A novel biochemical sensor. Chemtech, 12, 227–235.

    Google Scholar 

  • Bruner, L.H., Miller, R., Owicki, J.C., Parce, J.W., Muir, V.C. (1992). Testing ocular irritancy in vitro with the silicon microphysiometer. Toxic. In Vitro, 6, 272–284.

    Google Scholar 

  • Catroux, P., Rougier, A., Dossou, K.G., Cottin, M. (1993). The silicon microphysiometer for testing ocular toxicity in vitro. Toxic. In Vitro, 7, 465–469.

    Article  CAS  Google Scholar 

  • Cottin, M., Catroux, P., Delabarre, I., Rougier, A., Dossou, K.G. (1992). Complementary of four cytotoxicity methods and their relevance for ocular safety assessment of surfactants. Toxicology Letters, 15, 237.

    Google Scholar 

  • Grattarola, M., Martinola, S., Melani, M., Tedesco, M., Parodi, M.T. (1993). On-line extracellular pH measurements in culture as a tool for cell metabolism monitoring. STP. Pharma. Sciences, 3, 31–34.

    CAS  Google Scholar 

  • Hirst, M.A., Pitchford, S. (1993). Use of a single assay system to assess functionnal coupling of a variety of receptors. The Journal of NIH Research, 5, 69.

    Google Scholar 

  • McConnell, H.M., Rice, P., Wada, G.H., Owicki, J.C., Parce, J.W. (1991). The microphysiometer biosensor. Current opinion in structural biology, 1, 647–652.

    Article  CAS  Google Scholar 

  • Miller, D.L., Olson, J.C., Parce, J.W., Owicki, J.C. (1993). Cholinergic stimulation of the Na+/K+ ademosine triphosphatase as revealed by microphysiometry. Biophys. J., 64, 813–823.

    Article  PubMed  CAS  Google Scholar 

  • Nag, B., Wada, H.G., Fok, K.S., Green, D.J., Sharma, S.D., Clark, B.R., Parce, J.W., McConnell, H.M. (1992). Antigen-specific stimulation of T-cell extracellular acidification by MHC class II - peptide complexes. J. of Immunology, 148, 2040–2044.

    CAS  Google Scholar 

  • Owicki, J.C., Parce, J.W., Kercso, K.M., Sigal, G.B., Muir, V.C., Venter, J.C., Fraser, C.M., McConnell, H.M. (1990). Continuous monitoring of receptor-mediated changes in the metabolic rates of living cells. Proc. Natl. Acad. Sci., 87, 4007–4011.

    Article  PubMed  CAS  Google Scholar 

  • Owicki, J.C., Parce, J.W. (1992). Biosensors based on the energy metabolism of living cells: the physical chemistry and cell biology of extracellular acidification. Biosensors and bioelectronics, 7, 255–272.

    Article  PubMed  CAS  Google Scholar 

  • Parce, J., Owicki, J., Kercso, K., Sigal, G., Wada, VC., Muir, L., Bousse, L., Ross, K., Sikic, B., Mc. Connell, HM. (1989). Detection of cell affecting agents with a silicon biosensor. Sciences (Wash.DC), 240, 1182–1185.

    Google Scholar 

  • Parce, J.W., Owicki, J.C., Kercso, K.M. (1990). Biosensors for directly measuring cell affecting agents. Ann. Biol. Clin., 48, 639–641.

    CAS  Google Scholar 

  • Pitchford, S., Glaeser, B.S., De Moor, K. Rapid measurements of response by PC 12 cells to nerve growth factor (NGF) using the cytosensor microphysiometer system, (unpublished)

    Google Scholar 

  • Raley-Susman, K.M., Miller, K.R., Owicki, J.C., Sapolsky, R.M. (1992). Effects of excitotoxin exposure on metabolic role of primary hippocampal cultures: application of silicon microphysiometry to neurobiology. Journal of Neuro science, 12, 3, 773–780.

    CAS  Google Scholar 

  • Rosser, M.P., Kozlowski, M.R., Neve, R.U., Neve, K.A. (1991) Effects of D2 and D3 receptor activation measured by microphysiometry. Soc. Neurosci. ( Abs ), 17, 818.

    Google Scholar 

  • Salon, J.A., Johnson, R.A., Civelli, O. (1991) Real-time measurements of human Di and D2 receptor activity with a silicon-based biosensor. Soc. Neurosci. ( Abs ), 17, 86.

    Google Scholar 

  • Wada, H.G., Indelicato, S.R., Meyer, L., Kitamura, T., Miyajima, A., Kirk, G., Muir, V.C., Parce, J.W. (1993). GM-CSF triggers a rapid glucose dependant extracellular acidification by TF1 cells. Evidence for a sodium/proton antiporter and PKC mediated activation of acid production. J. Cell. Physiol, 154, 129–148.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Catroux, P., Cottin, M., Rougier, A., Leclaire, J. (1995). Biosensors in pharmacology and toxicology in vitro. In: Galli, C.L., Marinovich, M., Goldberg, A.M. (eds) Modulation of Cellular Responses in Toxicity. NATO ASI Series, vol 93. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79872-6_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-79872-6_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79874-0

  • Online ISBN: 978-3-642-79872-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics