Abstract
The polymerase chain reaction (PCR) is an in vitro technique allowing the amplification of specific DNA subsequences by simultaneous primer extension carried out by a heat-stable DNA polymerase added to the reaction mixture. Now performed in almost every modern laboratory, PCR has become a powerful and sensitive tool in biomedical research and one of the most widely used techniques in mRNA analysis.
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References
Taylor GR (1991) Polymerase chain reaction: basic principles and automation. In: McPherson MJ, Quirke P, Taylor GR (eds.): PCR: A practical approach. Practical approach series, Oxford University Press, New York, pp 1 -14
Erlich HA (ed.) (1989) PCR technology. Principles and applications for DNA amplification. MacMillan Publishers, London
Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds.) (1989) PCR protocols. A guide to methods and applications. Academic Press Inc., Harcourt Brace, Jovanovich Publishers, San Diego
Van der Bliek AM, Borst P (1989) Multidrug resistance. In: Advances in Cancer Research, Vol. 52. Academic Press, New York, pp. 165–203
McLean S, Hill BT (1992) An overview of membrane, cytosolic and nuclear proteins associated with the expression of resistance to multiple drugs in vitro. Biochim Biophys Acta; 1114:107–127
List AF, Spier C, Greer J, Wolf S, Hutter J, Dorr R, Salmon S, Futscher B, Baier M, Dalton W (1993) Phase I/II trial of cyclosporine as a chemotherapy-resistance modifier in acute leukemia. J Clin Oncol; 11:1652 -1660
Gekeler V, Frese G, Noller A, Handgretinger R, Wilisch A, Schmidt H et al. (1992) Mdrl/ P-glycoprotein, topoisomerase, and glutathione-S-transferase p gene expression in primary and relapsed state adult and childhood leukemias. Br J Cancer; 66:507–517
Kohler T, Lafiner D, Rost A-K, Leiblein S, Remke H (in press) Polymerase chain reaction related approaches to quantitate absolute levels of mRNA coding for the multidrug resistance-associated protein and P-glycoprotein. In: Proceedings of the 2nd International Symposium “Drug resistance in Leukemia and Lymphoma”, March 6 - 8,1995, Amsterdam, “Advances in Blood Disorders” series, Harwood Academic Publishers
Morales MJ, Gottlieb DI (1993) A polymerase chain reaction-based method for detection and quantification of reporter gene expression in transient transfection assays. Anal Bio- chem; 210:188–194
Bebee RL, Thornton CG, Hartley JL, Rashtchian, A (1992) Contamination-free polymerase chain reaction: endonuclease cleavage and cloning of dU-PCR products. Focus; 14:53–56
Ruano G, Brash DE, Kidd KK (1991) PCR: the first few cycles. Amplifications; 7:1–4
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© 1995 Springer-Verlag Berlin Heidelberg
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Köhler, T. (1995). Qualitative RT-PCR: Amplification of Synthesized mdr-1 cDNA. In: Quantitation of mRNA by Polymerase Chain Reaction. Springer Labor Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79712-5_7
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DOI: https://doi.org/10.1007/978-3-642-79712-5_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-79714-9
Online ISBN: 978-3-642-79712-5
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