Adipocyte Differentiation is Dependent on the Induction of the Acyl-CoA Binding Protein
The 3T3-L1 cell line was selected from an established Swiss mouse 3T3 cell lines by virtue of its ability to differentiate into cells possessing the morphological and biochemical characteristics of adipose cells (Green and Kehinde, 1974). Preconfluent growing 3T3-L1 cells maintain a fibroblastic phenotype, but upon confluence treatment with an appropriate combination of adipogenic factors induces a highly synchronous adipose conversion (reviewed in Cornelius et al, 1994). Several lines of evidence have indicated that the transcription factor CCAAT/enhancer binding protein α (C/EBPα) plays a decisive role in the differentiation process (Samuelsson et al, 1991; Lin and Lane, 1992; Lin and Lane, 1994; Freytag et al, 1994).
KeywordsFatty Acid Synthesis Adipocyte Differentiation Antisense Orientation Triglyceride Synthesis Triglyceride Accumulation
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