Preparation of Buffers

  • Gerhard Isenberg
Part of the Springer Lab Manual book series (SLM)

Abstract

In general, buffers are made up according to two methods:
  1. a)

    mix a dissolved solution of buffer and adjust the pH by titrating with a strong counterion,

     
  2. b)

    mix a solution of buffer from 10 x concentrated stock solutions.

     

Keywords

Citrate Glycine Cysteine Thiol Hepes 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Gerhard Isenberg
    • 1
  1. 1.Lehrstuhl für Biophysik E22Technische Universität MünchenGarching bei MünchenGermany

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