Forensic Application of Two Short Tandem Repeat Systems: HUMTH01 and HUMvWA31
Over the past few years many laboratories worldwide have introduced analysis of DNA polymorphisms as a routine method for forensic purposes. In particular, VNTRs, investigated by PCR, have reached an important position after considerable development. At present laboratories are studying a further series of highly polymorphic loci whose polymorphism derives from a dimeric-trimeric-tetrameric tandem repeated core sequence, named short tandem repeats (STRs) (Weber and May 1989; Edwards et a1.1991). The aim of the present study was to investigate two of these STRs, HUMTH01 (Edwards et al. 1992) and HUMvWA31 (Kimpton et al. 1992) to evaluate:1) allele frequencies in an Italian population sample, and 2) their efficiency for personal identification on aged bloodstains from various substrates.
KeywordsShort Tandem Repeat Forensic Application Forensic Purpose Allele Ladder Restriction Fragment Length Polymorphism Typing
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Allen RC, Graves G, Budowle B (1989) Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Bio Tech 7: 736–744Google Scholar
Brinkmann B, Rand S, Bajanowsky T (1992) Forensic identification of urine samples. Int J Leg Med 105: 59–61CrossRefGoogle Scholar
Budowle B, Baechtel FS (1990) Modifications to improve effectiveness of restriction fragment length polymorphism typing. Appl Theor Electrophor 1: 181–187PubMedGoogle Scholar
Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the variable number of tandem repeats locus D1S80 by the polymerase chain reaction followed by high resolution polyacrylamide gel electrophoresis. Am J Hum Genet 48: 137–144PubMedGoogle Scholar
Edwards A, Civitello A, Hammond HA, Caskey CT(1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49: 746–756PubMedGoogle Scholar
Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992) Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics 12: 241–253PubMedCrossRefGoogle Scholar
Kimpton C, Walton A, Gill P (1992) A further tetranucleotide repeat polymorphism in the vWF gene. Hum Mol Genet 1: 287.PubMedCrossRefGoogle Scholar
Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506–513PubMedGoogle Scholar
Weber J L and May P E (1989) Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet 44: 388–396PubMedGoogle Scholar
Wiegand P, Bajanowski T, Brinkmann B (1993) DNA typing of debris from fingernails. Int J Leg Med 106: 81–83CrossRefGoogle Scholar
© Springer-Verlag Berlin Heidelberg 1995