Gene-targeting approaches in the study of cellular processes involved in growth or differentiation

Advances in the analysis of oncogenes, tumour-suppressor genes, cytokine/receptor systems and developmental control genes
  • Gen Yamada
  • Kazuhisa Sugimura
  • Edward T. Stuart
Part of the EJB Reviews 1994 book series (EJB REVIEWS, volume 1994)


Recent advances in embryonic stem cell (ES) technology have made it possible to introduce mutations into the mouse genome by homologous recombination and thereby selectively generate mutant mice. Genetically manipulated mutant mice have contributed enormously to our understanding of the molecular functions of isolated genes and the mechanisms of cell growth and differentiation, as well as increasing our understanding of diseases such as cancer. Here, we will briefly summarise the recent advances in gene-targeting studies with particular emphasis on growth factors and their receptors, oncogenes, tumour-suppressor genes and developmental control genes.


Embryonic Stem Cell Nerve Growth Factor Mutant Mouse Homeobox Gene Embryonic Carcinoma Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


ES cell

embryonic stem cell

EC cell

embryonic carcinoma cell


nerve growth factor


brain-derived neurotrophin factor




ciliary neurotrophic factor


leukaemia inhibitory factor




oncostatin M


granulocyte macrophage-colony-stimulating factor


macrophage-colony-stimulating factor


central nervous system




neurofibromatosis 1


epidermal growth factor receptor


transforming growth factor.


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Copyright information

© FEBS 1994

Authors and Affiliations

  • Gen Yamada
    • 1
  • Kazuhisa Sugimura
    • 2
  • Edward T. Stuart
    • 1
  1. 1.Department of Molecular Cell BiologyMax Planck Institute of Biophysical ChemistryGöttingenGermany
  2. 2.Department of Applied Chemistry and Chemical EngineeringKagoshima UniversityJapan

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