Gene-targeting approaches in the study of cellular processes involved in growth or differentiation

Advances in the analysis of oncogenes, tumour-suppressor genes, cytokine/receptor systems and developmental control genes
  • Gen Yamada
  • Kazuhisa Sugimura
  • Edward T. Stuart
Part of the EJB Reviews 1994 book series (EJB REVIEWS, volume 1994)

Abstract

Recent advances in embryonic stem cell (ES) technology have made it possible to introduce mutations into the mouse genome by homologous recombination and thereby selectively generate mutant mice. Genetically manipulated mutant mice have contributed enormously to our understanding of the molecular functions of isolated genes and the mechanisms of cell growth and differentiation, as well as increasing our understanding of diseases such as cancer. Here, we will briefly summarise the recent advances in gene-targeting studies with particular emphasis on growth factors and their receptors, oncogenes, tumour-suppressor genes and developmental control genes.

Keywords

Recombination Influenza Sarcoma Stein Tetracycline 

Abbreviations

ES cell

embryonic stem cell

EC cell

embryonic carcinoma cell

NGF

nerve growth factor

BDNF

brain-derived neurotrophin factor

NT

neurotrophin

CNTF

ciliary neurotrophic factor

LIF

leukaemia inhibitory factor

IL

interleukin

OSM

oncostatin M

GM-CSF

granulocyte macrophage-colony-stimulating factor

M-CSF

macrophage-colony-stimulating factor

CNS

central nervous system

Rb

retinoblastoma

NF1

neurofibromatosis 1

EGF-R

epidermal growth factor receptor

TGF

transforming growth factor.

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Copyright information

© FEBS 1994

Authors and Affiliations

  • Gen Yamada
    • 1
  • Kazuhisa Sugimura
    • 2
  • Edward T. Stuart
    • 1
  1. 1.Department of Molecular Cell BiologyMax Planck Institute of Biophysical ChemistryGöttingenGermany
  2. 2.Department of Applied Chemistry and Chemical EngineeringKagoshima UniversityJapan

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