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Unlinking of DNA by Topoisomerases During DNA Replication

  • Chapter
Nucleic Acids and Molecular Biology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 9))

Abstract

The double-helical structure of DNA first described by Watson and Crick suggested immediately a mechanism for DNA replication (Watson and Crick 1953a,b). The two strands of the double helix need to unpair, after which they could serve as a template for the synthesis of complementary strands. The plectonemic, or interwound, nature of the double helix imposes a strict requirement on the mechanism of replication. The parental strands must be unlinked for the separation of daughter chromosomes to occur. Watson and Crick recognized that the problem was difficult, but “not insuperable” (Watson and Crick 1953a). It was difficult enough, however, to lead some early theorists to solutions involving breakage at every 1/2 turn of the helix during replication (Delbrück 1954) or a paranemic (side-by-side) structure for DNA instead of the plectonemic Watson and Crick model (Rodley et al. 1976; Pohl and Roberts 1978; Crick et al. 1979).

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, University of California-Berkeley, 401 Barker Hall, Berkeley, CA, 94720, USA

    C. J. Ullsperger, A. V. Vologodskii & N. R. Cozzarelli

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  2. A. V. Vologodskii
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  3. N. R. Cozzarelli
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  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, Dundee, DD1 4HN, UK

    David M. J. Lilley

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Ullsperger, C.J., Vologodskii, A.V., Cozzarelli, N.R. (1995). Unlinking of DNA by Topoisomerases During DNA Replication. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79488-9_6

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  • DOI: https://doi.org/10.1007/978-3-642-79488-9_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79490-2

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