Analysis of cerebrospinal fluid of patients with parkinsonism by proton nuclear magnetic resonance spectroscopy
High-resolution proton magnetic resonance spectroscopy was used to study the concentrations of metabolites in the cerebrospinal fluid (CSF) of patients with parkinsonism. The results indicated that the glucose concentration was significantly lower than in that of a normal control group; the lactate-to-glucose ratio was higher for the parkinsonism group, as was the citrate concentration. Other metabolites detected by proton nuclear magnetic resonance (NMR), such as lactate, acetate, creatine, glutamine and alanine, did not show significant differences between the parkinsonism and normal group. Our findings suggest that glucose is used more in the brain of patients with parkinsonism and therefore results in a higher lactate-to-glucose ratio. The higher level of citrate possibly indicated a disorder of the TCA cycle or acetyl CoA transfer in the so-called citratepyruvate cycle during fatty acids synthesis in certain areas of brain.
Key wordsParkinsonism Metabolism Glucose Lactate Citrate Magnetic resonance spectroscopy
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