The Renal H-K-ATPase: Function and Expression

  • Charles S. Wingo
  • Xiaoming Zhou
  • Adam Smolka
  • Kirsten Madsen
  • C. Craig Tisher
  • Kevin A. Curran
  • W. Grady Campbell
  • Brian D. Cain
Conference paper
Part of the NATO ASI Series book series (NATO ASI, volume 89)

Abstract

Evidence from several laboratories indicates that the collecting duct (CD) possesses a mechanism of active potassium reabsorption coupled to apical proton secretion. This process exhibits many of the characteristics of proton and potassium transport observed in the parietal cells of the gastric mucosa, and the accumulated evidence is consistent with the hypothesis that this process is due to a proton-potassium- activated adenosinetriphosphatase (an H-K-ATPase).

Keywords

Bromide Adenosine Electrophoresis Luminal Ethidium 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Backman, K.A. and J.P. Hayslett. 1983. Role of the medularry collecting duct in potassium conservation. Pfligers Arch. 396:297–300.Google Scholar
  2. Cannon, C., J. Van Adelsberg, S. Kelly, and Q. Al-Awqati. 1985. Carbon-dioxide-induced exocytotic insertion of H+ pumps in turtle-bladder luminal membrane: Role of cell pH and calcium. Nature 314: 443–446.CrossRefPubMedGoogle Scholar
  3. Cheval, L., C. Barlet-Bas, C. Khadouri, E. Feraille, S. Marsy, and A. Doucet. 1991. K+-ATPase-mediated Rb+ transport in rat collecting tubule: modulation during K+ deprivation. Am. J. Physiol Renal,Fluid Electrolyte Physiol 29: F800–F805.Google Scholar
  4. Crowson, M.S. and G.E. Shull. 1992. Isolation and characterization of a cDNA encoding the putative distal colon H+,K+-ATPase. Similarity of deduced amino acid sequence to gastric H+,K+-ATPase and Na+,K+-ATPase and mRNA expression in distal colon, kidney, and uterus./. Biol Chem. 267: 13740 - 13748.Google Scholar
  5. Curran, K.A., M.J. Hebert, B.D. Cain, and C.S. Wingo. 1992. Evidence for the presence of a K-dependent acidifying adenosine triphosphatase in the rabbit renal medulla. Kidney Int. 42: 1093 - 1098.CrossRefPubMedGoogle Scholar
  6. Curran, K.A, I.K. Hornstra, R. Cruz, L.G. McMahon, C.S. Wingo, and B.D. Cain. 1992. Partial cDNA clone of an apparent renal H-K-ATPase ß subunit. J. Am. Soc. Nephrol 3:775. Abstract.Google Scholar
  7. Doucet, A. and S. Marsy. 1987. Characterization of K-ATPase activity in distal nephron: Stimulation by potassium depletion. Am. J. Physiol Renal,Fluid Electrolyte Physiol 253:F418+F423.Google Scholar
  8. Garg, L.C. and N. Narang. 1988. Ouabain-insensitive K-adenosine triphosphatase in distal nephron segments of the rabbit. J. Clin Invest 81 (4): 1204–1208.CrossRefPubMedPubMedCentralGoogle Scholar
  9. Hansen, G.T., C.C. Tisher, and R.R. Robinson. 1980. Response of the collecting duct to disturbances of acid base and potassium balance. Kidney Int. 17: 326–337.CrossRefPubMedGoogle Scholar
  10. Linas, S.L., L.N. Peterson, R.J. Anderson, G.A Aisenbery, F.R. Simon, and T. Berl. 1979. Mechanism of renal potassium conservation in the rat. Kidney Int. 15: 601–611.CrossRefPubMedGoogle Scholar
  11. Madsen, K.M. and C.C. Tisher. 1983. Cellular response to acute respiratory acidosis in rat medullary collecting duct. Am. J. Physiol Renal,Fluid Electrolyte Physiol 245.-F670–F679.Google Scholar
  12. McKinney, T.D. and K.K. Davidson. 1988. Effects of respiratory acidosis on HCO3 transport by rabbit collecting tubules. Am. J. Physiol Renal, Fluid Electrolyte Physiol 255: F656 - F665.Google Scholar
  13. Perrone, R.D. and D.E. McBride. 1988. Aldosterone and pC02 enhance rubidium absorption in rat distal colon. Am. J. Physiol Gastrointest. Liver Physiol 17: G898–G906.Google Scholar
  14. Reuben, M.A., L.S. Lasater, and G. Sachs. 1990. Characterization of a B subunit of the gastric H+/K+-transporting ATPase. Proc. Natl Acad Sci U.S.A. 87: 6767–6771.CrossRefPubMedPubMedCentralGoogle Scholar
  15. Reuben, M.A., F.L. Starr, S. Birmingham, G. Sachs, and J.A Kraut. 1993. Characterization of a renal H,K ATPase alpha subunit mRNA found to be identical with gastric H,K ATPase mRNA Gastroenterology 104:A177. Abstract.Google Scholar
  16. Silver, R.B. and G. Frindt. 1993. Functional identification of H-K-ATPase in intercalated cells of cortical collecting tubule. Am. J. Physiol Renal, Fluid Electrolyte Physiol 264: F259–F266.Google Scholar
  17. Spargo, B. 1954. Kidney changes in hypokalemic alkalosis in the rat. J. Lab. Clin. Med. 43: 802–814.PubMedGoogle Scholar
  18. Stetson, D.L., J.B. Wade, and G. Giebisch. 1980. Morphologic alterations in the rat medullary collecting duct following potassium depletion. Kidney Int. 17: 45–56.CrossRefPubMedGoogle Scholar
  19. Van Adelsberg, J. and Q. Al-Awqati. 1986. Regulation of cell pH by Ca+2-mediated exocytotic insertion of H+-ATPase. J. Cell Biol 102: 1638 - 1645.CrossRefPubMedGoogle Scholar
  20. Wingo, C.S. 1987. Potassium transport by the medullary collecting tubule of rabbit: effects of variation in K intake. Am. J. Physiol Renal, Fluid Electrolyte Physiol 22: F1136–F1141.Google Scholar
  21. Wingo, C.S. 1989. Active proton secretion and potassium absorption in the rabbit outer medullary collecting duct - functional evidence for proton-potassium activated adenosine triphosphatase. J. Clin Invest. 84: 361–365.CrossRefPubMedPubMedCentralGoogle Scholar
  22. Wingo, C.S. and F.E. Armitage. 1992. Rubidium absorption and proton secretion by rabbit outer medullary collecting duct via H-K-ATPase. Am. J. Physiol Renal,Fluid Electrolyte Physiol 263: F849–F857.Google Scholar
  23. Wingo, C.S., K.M. Madsen, A Smolka, and C.C. Tisher. 1990. H-K-ATPase immunoreactivity in cortical and outer medullary collecting duct. Kidney Int. 38: 985–990.CrossRefPubMedGoogle Scholar
  24. Zhou, X. and C.S. Wingo. 1992. Mechanisms for enhancement of Rb efflux by 10% C02 in cortical collecting duct (CCD). Clin. Research 40:179A Abstract.Google Scholar
  25. Zhou, X. and C.S. Wingo. 1992. Mechanisms of rubidium permeation by rabbit cortical collecting duct during potassium restriction. Am. J. Physiol Renal, Fluid Electrolyte Physiol 263: F1134–F1141.Google Scholar
  26. Zhou, X. and C.S. Wingo. 1992. H-K-ATPase enhancement of Rb efflux by cortical collecting duct. Am. J. Physiol. Renal, Fluid Electrotype Physiol. 32:F43–F48.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Charles S. Wingo
    • 1
    • 2
  • Xiaoming Zhou
    • 2
  • Adam Smolka
    • 3
  • Kirsten Madsen
    • 1
  • C. Craig Tisher
    • 1
  • Kevin A. Curran
    • 1
  • W. Grady Campbell
    • 4
  • Brian D. Cain
    • 4
  1. 1.Division of Nephrology, Hypertension and TransplantationUniversity of FloridaGainesvilleUSA
  2. 2.Department of PhysiologyUniversity of FloridaGainesvilleUSA
  3. 3.Division of GastroenterologyMedical University of South CarolinaCharlestonUSA
  4. 4.Department of Biochemistry and Molecular BiologyUniversity of FloridaGainesvilleUSA

Personalised recommendations