Extrachromosomal Inheritance: Yeast Linear Killer Plasmids as a Tool in Genetic Engineering

Schründer, Jürgen; Meinhardt, Friedhelm

doi:10.1007/978-3-642-79249-6_15

Extrachromosomal Inheritance: Yeast Linear Killer Plasmids as a Tool in Genetic Engineering

Jürgen Schründer⁶ &
Friedhelm Meinhardt⁶

Chapter

231 Accesses
2 Citations

Part of the book series: Progress in Botany/Fortschritte der Botanik ((BOTANY,volume 56))

Abstract

In principle, every stably replicated DNA species can serve as a vector for cloning foreign DNA in yeast or any other organism, in which such DNA is found. In strains of Saccharomyces cerevisiae a high copy number, autonomously replicating, extrachromosomal DNA element, the 2 µm DNA, was found to exist (Sinclair et al. 1967). This covalently closed circular DNA element resembles in many respects bacterial plasmids. Since it is present in approximately 50 copies per cell it is relatively stably maintained. It was used to construct vectors for cloning and expression of foreign genes in Saccharomyces cerevisiae (for review see Hollenberg 1982). The occurrence of circular plasmids is not restricted to baker’s yeast, because similar elements were also detected in Zygosaccharomyces (Tohe and Utatsu 1985) and in Kluyveromyces drosophilarum (Chen et al. 1986). The latter plasmid, pKD1, is 1.6 µm in size and was also transferred and stably maintained in other yeast species of the genus, i.e., Kluyveromyces lactis (Bianchi et al. 1987).

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Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Correnstraße 3, 48149, Münster, Germany
Dipl. Biol. Jürgen Schründer & Prof. Dr. Friedhelm Meinhardt

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Dipl. Biol. Jürgen Schründer
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Prof. Dr. Friedhelm Meinhardt
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Zellenlehre, Universität Heidelberg, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany
H.-Dietmar Behnke
Institut für Botanik, FB Biologie (10), TH Darmstadt, Schnittspahnstraße 3-5, 64287, Darmstadt, Germany
Ulrich Lüttge
Lehrstuhl für Allgemeine Botanik, Ruhr Universität, Postfach 10 21 48, 44780, Bochum 1, Germany
Karl Esser
Institut für Spezielle Botanik und Botanischer Garten, Universität Mainz, Saarstraße 21, 55099, Mainz, Germany
Joachim W. Kadereit
Lehrstuhl für Geobotanik, Systematisch-Geobotanisches Institut der Universität, Untere Karspüle 2, 37073, Göttingen, Germany
Michael Runge

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Schründer, J., Meinhardt, F. (1995). Extrachromosomal Inheritance: Yeast Linear Killer Plasmids as a Tool in Genetic Engineering. In: Behnke, HD., Lüttge, U., Esser, K., Kadereit, J.W., Runge, M. (eds) Progress in Botany. Progress in Botany/Fortschritte der Botanik, vol 56. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79249-6_15

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DOI: https://doi.org/10.1007/978-3-642-79249-6_15
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