The Preparation and Culturing of Rat Hepatocytes
Isolated, cultured hepatocytes would seem at first sight to be an ideal model system for the study of hepatocarcinogenesis and the testing of chemicals that may be carcinogens as the liver is the major site of activation of many potential carcinogens and, thus can act as generator of the toxic metabolite as well as the target.The great potential of this model is, however, diluted by the diversity of approaches taken by various workers in the field to the preparation and culturing of hepatocytes.There is no accepted or recommended method for preparing the cells from intact liver (although many methods have been published) and little work on the optimisation of the medium and medium additions needed for culturing of hepatocytes.
KeywordsToxicity Albumin Lactate Hydrocortisone Heparin
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