mRNA Composition of Rat Liver Tumors Initiated by Aromatic Amines

  • Annette Bitsch
  • Martina Jost
  • Hedwig Richter
Conference paper
Part of the NATO ASI Series book series (volume 88)

Abstract

Many polycyclic aromatic amines are mutagenic. Their genotoxic properties are explained by the formation of metabolites that react with DNA by forming adducts (Kriek 1969, Franz et al. 1986). These DNA adducts may cause mutations which are responsible for tumorigenic effects. The carcinogenesis induced by aromatic amines is very often characterized by high tissue specificity. Chronic feeding of 2-acetylaminofluorene (AAF) typically produces liver tumors in rodents, 2-acetylaminophenanthrene (AAP) mammary tumors and trans-4-acetylaminostilbene (AAS) Zymbal’s gland tumors (Neumann et al. 1970). Many attempts have been made to correlate DNA binding or the resulting lesions with tissue specific tumor formation. However, this phenomenon cannot be readily explained by the extent of DNA modifications. All three amines generate comparable DNA adduct levels in rat liver and moreover in liver more extensively than in any other tissue (Neumann 1983, Ruthsatz and Neumann 1988, Gupta et al. 1989). Therefore, DNA binding may reflect the formation of some critical lesions related to tumor initiation but is not sufficient to explain carcinogenic effects. AAS and AAP are able to produce liver tumors in rats, but only if initiation is followed by some promotion treatment (Hammerl 1989). AAF in contrast is a complete carcinogen for this tissue.

Keywords

Electrophoresis Adduct Oncol Aflatoxin Toxicology 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Annette Bitsch
    • 1
  • Martina Jost
    • 1
  • Hedwig Richter
    • 1
  1. 1.Institute of Pharmacology and ToxicologyUniversity of WürzburgWurzburgGermany

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