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13C-1H Spin-Coupling Constants in Furanosyl Rings: Application to Oligonucleotide Structure Determination

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NMR of Biological Macromolecules

Part of the book series: NATO ASI Series ((ASIH,volume 87))

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Abstract

Conformational analysis of the five-membered (furanose) rings of oligonucleotides commonly relies on the use of three-bond (vicinal) 1H-1H spin-coupling constants (3JHH) obtained from an analysis of 2D NMR cross-peaks (Schmitz et al 1990; Emsley et al 1993; Majumdar and Hosur 1992). For a HaCxCyHb fragment, the magnitude of 3JHa,Hb depends on the Cx-Cy torsion angle, with maximal coupling observed when Ha and Hb are eclipsed or antiperiplanar, and minimal coupling observed when these atoms are approximately orthogonal (Karplus 1959); in the ensuing years since this discovery, more refined Karplus relationships have been proposed that account for substitution and/or electronegativity effects at Cx and Cy on 3JHa,Hb (Haasnoot et al 1980).

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, 46556, USA

    Anthony S. Serianni

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  1. Anthony S. Serianni
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Editors and Affiliations

  1. Institute of Biology, National Centre for Scientific Research ”Demokritos“, 153 10, Ag. Paraskevi, Greece

    Chariklia Ioannidou Stassinopoulou

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© 1994 Springer-Verlag Berlin Heidelberg

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Serianni, A.S. (1994). 13C-1H Spin-Coupling Constants in Furanosyl Rings: Application to Oligonucleotide Structure Determination. In: Stassinopoulou, C.I. (eds) NMR of Biological Macromolecules. NATO ASI Series, vol 87. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79158-1_16

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  • DOI: https://doi.org/10.1007/978-3-642-79158-1_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79160-4

  • Online ISBN: 978-3-642-79158-1

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