Structural and Conformational Studies of Glycoproteins and Oligosaccharide Recognition Determinants
Oligosaccharide chains often constitute a large proportion of the molecular mass of glycoproteins. Their relative hydrophilicity, flexibility and heterogeneity can make crystallisation difficult or impossible for X-ray studies of the native molecule and hence NMR spectroscopy is the method of choice for analysis. NMR is also useful for analysis of oligosaccharide sequences found on glycoproteins, glycolipids, proteoglycans, polysaccharides, antibiotics and as free oligosaccharides which are recognition determinants and potential drugs. Their multiple chiral centres and polyhydroxyl functionality make oligosaccharides important natural information molecules and artificial templates on which to stereochemically design ligands for different protein or DNA binding motifs.
KeywordsHeparin Polysaccharide Lactose Disaccharide Glycopeptide
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