Regulation of Gene Expression in Response to Drought and Osmotic Shock

  • Stefania Grillo
  • Antonello Costa
  • Marina Tucci
  • Antonella Leone
Conference paper
Part of the NATO ASI Series book series (volume 86)

Abstract

We have studied the response of potato cell suspension culture to PEGmediated low water potential. Gradual adaptation to high concentrations of PEG 8000 (20%) did not affect growth and endogenous ABA content of potato cells. On the contrary, direct exposure to 20% PEG (osmotic shock) drastically reduced growth in unadapted cells and also induced a six-fold increase in ABA level within 5 days from the imposition of the stress. 2D-electrophoretic pattern of in vivo labeled proteins of adapted cells was very similar to’ that of control cells. Only few polypeptides were down-regulated and the synthesis of at least 31 individual polypeptides was increased upon adaptation. Osmotic shock as well as ABA treatment of unadapted cells determined an overall reduction of protein synthesis. Most of the induced polypeptides in PEG-shocked cells were also found to be induced in unadapted cells upon treatment with exogenous ABA. However, it was possible to identify some polypeptides specifically induced in osmotically shocked cells, a group of which was also detected in adapted cells. The comparison of protein electrophoretic patterns demonstrated t h a t most changes observed in PEG adapted cells were not mediated by ABA. Long-term adaptation of potato cells to PEG enhanced both the transcript and protein level of osmotin, a 26 kD protein reported to accumulate in salt-adapted tobacco cells. In osmotic shocked cells a dramatic increase of osmotin transcripts was also detected, which however did not correlate with the level of t h e protein.

Keywords

Glycerol Acetone Phenol Urea Agarose 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Stefania Grillo
    • 1
  • Antonello Costa
    • 2
  • Marina Tucci
    • 2
  • Antonella Leone
    • 1
  1. 1.Research Center for Vegetable BreedingCNRPorticiItaly
  2. 2.Department of Agronomy and Plant GeneticsUniversity of NaplesPorticiItaly

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