Extraction of DNA and RNA from Aquatic Environments

  • J. H. Paul
  • S. L. Pichard
Part of the Springer Lab Manuals book series (SLM)


The capability to work with nucleic acids isolated from mixed microbial communities from aquatic environments opens a new avenue of investigation for understanding the structure and function of aquatic microbial communities. These technologies have enabled microbial ecologists to answer questions that could not be answered by the classical isolation and cultivation approach to aquatic microbiology. In the first part of this chapter, we review literature on the extraction and quantitation of nucleic acids from aquatic environments as biomass indicators. In the second part we discuss extraction of nucleic acids (both dissolved and particulate) for molecular biological purposes and provide protocols that are useful for this purpose.


Sodium Dodecyl Sulfate Aquatic Environment Viral Abundance Natural Microbial Community Guanidinium Isothiocyanate 
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© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • J. H. Paul
  • S. L. Pichard

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