Abstract
DNA-damaging effects of asbestos in the presence of hydrogen-peroxide, organic peroxides and hydroperoxides were investigated. The DNA damage was assessed in terms of the destabilization of secondary structure of DNA, damage to deoxyribose sugar and DNA fidelity which were measured respectively by S-1 nuclease hydrolysis, formation of thiobarbituric acid (TBA) — reacting species and melting temperature (Tm) profile using calf thymus DNA. S-1 nuclease hydrolysis and Tm determinations have shown that the presence of hydrogen peroxide (H2O2), benzoyl peroxide (BOOB), Cumene hydroperoxide (COOH) or tertiarybutyl hydroperoxide (t-BOOH) augmented asbestos-mediated DNA damage many folds compared to either asbestos alone or H202/peroxide/hydroperoxide alone. H2O2 also damaged deoxyribose sugar in DNA whereas no formation of TBA-reacting species could be observed in case of any other peroxide/hydroperoxide. The quenchers of reactive oxygen species (ROS) afforded protection against these DNA damage. These results suggest that asbestos in the presence of H2O2/organic peroxides damages DNA which is mediated by the generation of oxygen free radicals. The implications have been made for the significance of these results in relation to the observed development of cancer of respiratory tract among the asbestos-exposed population.
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Rahman, Q., Mahmood, N., Khan, S.G., Athar, M. (1994). Augmentation in the Differential Oxidative DNA-Damage by Asbestos in Presence of H2O2 and Organic Peroxide/Hydroperoxide. In: Davis, J.M.G., Jaurand, MC. (eds) Cellular and Molecular Effects of Mineral and Synthetic Dusts and Fibres. NATO ASI Series, vol 85. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79041-6_15
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DOI: https://doi.org/10.1007/978-3-642-79041-6_15
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