Platelet von Willebrand Factor — Collagen Binding Activity and Ristocetin Cofactor Activity: A Comparison of Methods Able to Differentiate von Willebrand Disease Types I and II (Preliminary Results)
von Willebrand disease (vWD) is the most common inherited bleeding disorder. By laboratory analysis vWD affects approximately 1% of the general population. Clinically significant vWD occurs in approximately 125 per million population [1,2]. The disease is caused by the quantitative absence or qualitative alteration of von Willebrand Factor (vWF) — a multimeric glycoprotein present in plasma and platelets, synthesised in endothelial cells and in megakaryocytes . Classification of patients with vWD into subtypes is important for the correct choice of treatment as well as for genetic counselling. The patient’s clinical symptoms, heredity patterns and the laboratory results of vWF analysis vary depending on the vWD subtype . The bleeding symptoms in patients with vWD are believed to be due to an abnormal interaction of vWF with the subendothelium and/or with the platelets during formation of the haemostatic plug, illustrating the main physiological roles of vWF during normal haemostasis [5–7].
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