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Microbial Mats pp 173-182 | Cite as

Light and electron microscopy in microbial mat research: An overview

  • John F. Stolz
Part of the NATO ASI Series book series (volume 35)

Abstract

A variety of traditional techniques for light microscopy (e.g., differential interference contrast, phase contrast, fluorescence) and electron microscopy (transmission and scanning) have been used to obtain valuable information on the structure and species composition of microbial mat communities. Recent advances in techniques and instrumentation for microscopy are providing new tools for studying these communities in situ. Innovations in immunohistochemistry and molecular biology allow in situ labeling of specific microorganisms with fluorophores or gold particles which have been conjugated to antibodies or oligonucleotides. Digital image analysis software and confocal laser microscopy have made serial sections and 3-D reconstruction possible, providing structural data on biofilms, microbial mats, and microfossils in petrographic sections. This review will highlight a few examples of these new technologies with emphasis on those which have potential use in microbial mat studies.

Keywords

Phototrophic Bacterium Magnetotactic Bacterium Benthic Microbial Community Digital Image Analysis Software Microcoleus Chthonoplastes 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • John F. Stolz
    • 1
  1. 1.Department of Biological SciencesDuquesne UniversityPittsburghUSA

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