Skip to main content

Signal Transduction and Myofibrillogenesis in Isolated Neonatal Heart Myocytes In Vitro

  • Conference paper
Biomechanics of Active Movement and Division of Cells

Part of the book series: NATO ASI Series ((ASIH,volume 84))

  • 180 Accesses

Abstract

A precise sequence of morphological events is associated with the process of myofibrillogenesis in vitro in neonatal cardiac myocytes (Hilenski et al 1991; Sharp et al 1993; Sanger et al 1984). A similar set of sequences probably occurs in vivo; however, the rate of formation is more gradual, thus making investigation of precise events more difficult. In vitro analyses of morphological and biochemical events associated with myofibrillogenesis can provide an understanding of these processes in normal heart development, response to stimulation, and cardiac hypertrophy.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bockholt SM and Burridge K (1993) Cell spreading on extracellular matrix proteins induces tryosine phosphorylation of tensin. J Biol Chem 268: 14565–14567.

    Google Scholar 

  • Borg TK and Caulfield JB (1981) The collagen materix of the heart. Fed Proc 40: 2037–2042.

    Google Scholar 

  • Borg TK, Rubin K, Lundgren E, Borg K, and Obrink B (1984) Recognition of extracellular matrix components by neonatal and adult cardiac myocytes. Dev Biol 104: 86–96

    Article  Google Scholar 

  • Burridge K, Fath K, Kelly G, Nucknolls G, and Turner C (1988) Focal adhesions transmembrane junctions between the extracellular matrix and the cytoskeleton. Ann Rev Cell Biol 4: 487–525.

    Article  Google Scholar 

  • Hilenski LL, Terracio L and Borg TK (1991) Myofibrillar and cytoskeletal assembly in neonatal rat cardiac myocytes cultured on laminin and collagen. Cell Tissue Res 264: 577–587

    Article  Google Scholar 

  • Hilenski LL, Xuehui M, Vinson N, Terracio L and Borg TK (1992) The role of β1 integrin in spreading and myofibrillogenesis in neonatal rat cardiomyocytes in vitro. Cell Motility and Cytoskel 21: 87–100

    Article  Google Scholar 

  • Hynes RO (1992) Integrinsversatility, modulation and signaling in cell adhesion. Cell 69: 11–25.

    Article  Google Scholar 

  • Juliano RL and Haskill S (1993) Signal transduction from the extracellular matrix. J Cell Biol 120: 577–585.

    Article  Google Scholar 

  • Robinson TF, Cohen-Gould L, and Factor SM (1983) Skeletal framwork of mammalian heart muscle. Arrangement of inter and pericellular connective tissue structures. Lab Invest 49: 482–495.

    Google Scholar 

  • Sanger JW, Mittal B, and Sanger JM (1984) Analysis of myofibrillar structure and assembly using fluorescently labeled contractile proteins. J Cell Biol 98: 825–834

    Article  Google Scholar 

  • Sharp WW, Terracio L, Borg TK and Samarel AM (1993) Contractile activity modulates actin synthesis and turnover in cultured neonatal rat heart cells. Circ Res 73: 172–183

    Google Scholar 

  • Terracio L, Rubin K, Jyring R, Gullberg D, Carver W and Borg TK (1991) Expression of collagen binding integrins during cardiac development and hypertrophy. Circ Res 68: 734–744.

    Google Scholar 

  • Terracio L, Simpson DG, Hilenski L, Carver W, Decker RS, Vinson N and Borg TK (1990) Distribution of vinculin in the Z-disk of striated muscle: Analysis by laser scanning confocal microscopy. J Cell Physiol 145: 78–87.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Sharp, W., Simpson, D.G., Terracio, L., Borg, T.K. (1994). Signal Transduction and Myofibrillogenesis in Isolated Neonatal Heart Myocytes In Vitro. In: AkkaÅŸ, N. (eds) Biomechanics of Active Movement and Division of Cells. NATO ASI Series, vol 84. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78975-5_31

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-78975-5_31

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78977-9

  • Online ISBN: 978-3-642-78975-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics