Locomoting Blebbing Cells: A New Model to Test Whether Formation of Protrusions is Primarily Due to Hydrostatic Pressure or to Actin Elongation
In blebbing cells the membrane is pushed forward faster (about 2.4 μm /sec) than known rates of actin elongation.
Immunofluorescent studies indicate that blebs show either diffuse staining for F-actin and α-actinin or only a linear staining along the cell membrane. These two patterns may represent different stages of bleb formation.
Formation of blebs or ruffles as well as the development of a polarized shape in neutrophils is suppressed in hypertonic media (0.5 M sorbitol).
KeywordsHydrostatic Pressure Actin Polymerization Locomote Cell Primary Force Bleb Formation
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