Stromal Function in Long Term Bone Marrow Culture of Patients with Acute Myeloid Leukemia
Inhibition of normal hemopoiesis-peripheral blood pancytopenia and complete absence of normal colony forming cells in bone marrow [3, 4], is a regular finding in acute (AML) myeloid leukemias . However, this inhibition is reversible. Polyclonal normal hemopoiesis is reestablished in more than two thirds of cases of AML during complete remission, demonstrating the existence of normal stem cells . A few pathogenic mechanisms have been proposed to account for this process: cell crowding, synthesis by leukemic cells of inhibitor substances, cell contact processes and deficiency in the stromal cell microenvironment . Several humoral inhibitors were found to be produced by leukemic cells such as acidic isoferritins, LAI and TNF-alpha [4, 5, 6]. Very little is known about the role of the microenvironment in the inhibition of normal hemopoiesis.
KeywordsAgar Leukemia Hydrocortisone Sedimentation Penicillin
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