Stromal Function in Long Term Bone Marrow Culture of Patients with Acute Myeloid Leukemia

  • Michail Ya. Lisovsky
  • Valeri G. Savchenko
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 37)

Abstract

Inhibition of normal hemopoiesis-peripheral blood pancytopenia and complete absence of normal colony forming cells in bone marrow [3, 4], is a regular finding in acute (AML) myeloid leukemias [1]. However, this inhibition is reversible. Polyclonal normal hemopoiesis is reestablished in more than two thirds of cases of AML during complete remission, demonstrating the existence of normal stem cells [15]. A few pathogenic mechanisms have been proposed to account for this process: cell crowding, synthesis by leukemic cells of inhibitor substances, cell contact processes and deficiency in the stromal cell microenvironment [2]. Several humoral inhibitors were found to be produced by leukemic cells such as acidic isoferritins, LAI and TNF-alpha [4, 5, 6]. Very little is known about the role of the microenvironment in the inhibition of normal hemopoiesis.

Keywords

Agar Leukemia Hydrocortisone Sedimentation Penicillin 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Michail Ya. Lisovsky
    • 1
  • Valeri G. Savchenko
    • 1
  1. 1.Dept. of Hematological Oncology and Bone Marrow TransplantationHematological Research CenterMoscowRussia

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