Abstract
Exposure of mammalian cells to restriction endonucleases (RE) leads to the induction of chromosomal aberrations (CA) and sister chromatid exchanges (SCE) (Obe et al. 1986; Obe and Johannes 1987; Natarajan and Mullenders 1987; Folle et al. 1992). The induction of CA and SCE by RE may suggest that the ultimate lesion for the production of chromosomal alterations is a DNA double-strand break (Obe et al. 1992; Bryant 1990; Morgan et al. 1990). RE induce CA independent of the S-phase of the cell cycle (Obe and Winkel 1985; Obe et al. 1987).
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References
Bianchi MS, Bianchi NO, Pantelias GE, Wolff S (1985) The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes. Chromosoma 91: 131–136
Bryant PE (1990) Restriction endonuclease and radiation-induced DNA double-strand breaks and chromosomal aberrations: Similarities and differences. In: Obe G and Natarajan AT (eds) Chromosomal Aberrations. Basic and Applied Aspects. Springer-Verlag, Berlin Heidelberg New York, pp. 61–69
Burkholder GD (1989) Morphological and biochemical effects of endonucleases on isolated chromosomes in vitro. Chromosoma 97: 347–355
Drets ME (1978) Bandscan–A computer program for on-line linear scanning of human banded chromosomes. Computer Programs in Biomedicine 8: 283–294
Drets ME, Seuanez H (1974) Quantitation of heterogeneous human heterochromatin: microdensitometric analysis of C- and G-bands. In: Coutinho EM and Fuchs F (eds) Physiology and Genetics of Reproduction. Basic Life Science, Vol. 4, part A,, Plenum Press, New York, pp 29–52
Drets ME, Monteverde FJ (1987) Automated cytogenetics with modern computerized scanning microscope photometer systems. In: Obe G and Basler A (eds) Cytogenetics. Basic and Applied Aspects. Springer-Verlag, Berlin Heidelberg New York, Chapter 3, pp. 48–64
Drets ME, Folle GA, Monteverde FJ (1989) Quantitative detection of chromosome structures by computerized microphotometric scanning. In: Obe G and Natarajan AT (eds) Chromosomal Aberrations. Basic and Applied Aspects. Springer-Verlag, Berlin Heidelberg New York, pp. 1–12
Drets ME, Obe G, Monteverde FJ, Folle GA, Medina II, De Galvez MG, Duarte JE, Mechoso BH (1992) Computerized graphic and light microscopic analyses of T-banded chromosome segments of Chinese hamster ovary cells and human lymphocytes. Biol Zbl 111: 204–214
Folle GA, Johannes C, Mechoso B, Medina II, Obe G (1992) The restriction endonuclease Alu I induces sister chromatid exchanges in Chinese hamster ovary cells. Mutagenesis 7: 291–294
Mezzanotte R, Bianchi U, Vanni R, Ferrucci L (1983) Chromatin organization and restriction nuclease activity on human metaphase chromosomes. Cytogenet Cell Genet 36: 562–566
Miller DA, Choi YC, Miller OJ (1983) Chromosome localization of highly repetitive human DNAs and amplified ribosomal DNA with restriction enzymes. Science 219: 395–397
Morgan WF, Winegar RA (1990) The use of restriction endonucleases to study the mechanisms of chromosome damage. In: Obe G and Natarajan AT (eds) Chromosomal Aberrations. Basic and Applied Aspects. Springer-Verlag, Berlin Heidelberg New York, pp. 70–78
Natarajan AT, Mullenders LHF (1987) Sister chromatid exchanges. In: Obe G and Basler A (eds) Cytogenetics. Basic and Applied Aspects. Springer-Verlag, Berlin Heidelberg New York, pp 338–344
Obe G, Winkel E-U (1985) The chromosome breaking activity of the restriction endonuclease Alu I in CHO cells is independent of the S-phase of the cell cycle. Mutation Res 152: 25–29
Obe G, Jonas R, Schmidt S (1986) The restriction endonuclease Alu I induces chromosomal aberrations in human peripheral lymphocytes in vitro. Mutation Res 150: 359–368
Obe G, Johannes C (1987) Chromosomal aberrations induced by the restriction endonucleases Alu I and Barn HI: Comparison with X-rays. Biol Zbl 106: 175–190
Obe G, Vasudev V, Johannes C (1987) Chromosome aberrations induced by restriction endonucleases. In: Obe G and Basler A (eds) Cytogenetics. Basic and Applied Aspects. Springer-Verlag, Berlin Heidelberg New York, Chapter 13, pp. 300–314
Obe G, Johannes C, Schulte-Frohlinde D (1992) DNA double-strand breaks induced by sparsely ionizing radiation and endonucleases as critical lesions for cell death, chromosomal aberrations, mutations and oncogenic transformation. Mutagenesis 7: 3–12
Piper J (1990) Automated cytogenetics in the study of mutagenesis and cancer. In: Obe G (ed) Advances in Mutagenesis Research. Springer-Verlag, Berlin Heidelberg New York, 2: 127–151
Ploem JS (1986) New instrumentation for sensitive image analysis of fluorescence in cells and tissues. Applications of fluorescence in the biomedical sciences. Alan R. Liss, New York, pp 289–300
Ray M, Mohandas T (1976) Proposed nomenclature for the Chinese hamster chromosomes (Cricetulus griseus). In: Hamerton JL. Report of the Committee on Chromosome Markers. Cytogen Cell Gen 16: 83–91
Seabright M (1971) A rapid banding technique for human chromosomes. Lancet II: 971–972
Siciliano MJ, Stallings RL, Adair GM (1985) The genetic map of the Chinese hamster and the genetic consequences of chromosomal rearrangements in CHO cells. In: Gottesman MM (ed) Molecular Cell Genetics. John Wiley & Sons, New York
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© 1994 Springer-Verlag Berlin Heidelberg
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Drets, M.E. et al. (1994). Quantitative Localization of Chromatid Breaks Induced by Alu I in the Long Arms of Chromosomes Number 1 of Chinese Hamster Ovary (CHO) Cells by Microphotometric Scanning. In: Obe, G., Natarajan, A.T. (eds) Chromosomal Alterations. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78887-1_18
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DOI: https://doi.org/10.1007/978-3-642-78887-1_18
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