Forensic application of SSCP in the analysis of HLA-DQ, which was amplified by PCR
Although various method have been developed for the confirmation of various alleles belonging to HLA class II genetic loci, SSO bot hybridization (1), RFLP (2), SSCP (3), SSCP/ARMS (4), some difficults are encountered always in forensic practice. Resently, we have succeeded in establishing method for diagnosis of HLA -DQA1 alleles by appling PCR-amplification and simple SSCP using a covenient primer set, which is recommend by International Histocompatibility Workshop(1991) (5). Based on the experience, we have examined the DNA analysis of alleles belonging to HLA- DQB and found the experimental results give comformity diagnosis of alleles belonging, not only to HLA-DQB1, but also -DQB2. This method can be used for the routine forensic test in determining identical identification.
KeywordsGlycerol EDTA Agarose Electrophoresis MgCl
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