Abstract
Although various method have been developed for the confirmation of various alleles belonging to HLA class II genetic loci, SSO bot hybridization (1), RFLP (2), SSCP (3), SSCP/ARMS (4), some difficults are encountered always in forensic practice. Resently, we have succeeded in establishing method for diagnosis of HLA -DQA1 alleles by appling PCR-amplification and simple SSCP using a covenient primer set, which is recommend by International Histocompatibility Workshop(1991) (5). Based on the experience, we have examined the DNA analysis of alleles belonging to HLA- DQB and found the experimental results give comformity diagnosis of alleles belonging, not only to HLA-DQB1, but also -DQB2. This method can be used for the routine forensic test in determining identical identification.
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© 1994 Springer-Verlag Berlin Heidelberg
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Akiyama, K., Yoshii, T., Ishiyama, I. (1994). Forensic application of SSCP in the analysis of HLA-DQ, which was amplified by PCR. In: Bär, W., Fiori, A., Rossi, U. (eds) Advances in Forensic Haemogenetics. Advances in Forensic Haemogenetics, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78782-9_89
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DOI: https://doi.org/10.1007/978-3-642-78782-9_89
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-57643-3
Online ISBN: 978-3-642-78782-9
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