Forensic application of SSCP in the analysis of HLA-DQ, which was amplified by PCR

  • Katsunori Akiyama
  • Tomio Yoshii
  • Ikuo Ishiyama
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

Although various method have been developed for the confirmation of various alleles belonging to HLA class II genetic loci, SSO bot hybridization (1), RFLP (2), SSCP (3), SSCP/ARMS (4), some difficults are encountered always in forensic practice. Resently, we have succeeded in establishing method for diagnosis of HLA -DQA1 alleles by appling PCR-amplification and simple SSCP using a covenient primer set, which is recommend by International Histocompatibility Workshop(1991) (5). Based on the experience, we have examined the DNA analysis of alleles belonging to HLA- DQB and found the experimental results give comformity diagnosis of alleles belonging, not only to HLA-DQB1, but also -DQB2. This method can be used for the routine forensic test in determining identical identification.

Keywords

Glycerol EDTA Agarose Electrophoresis MgCl 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  1. 1).
    Saiki, R.K., Bugawa, T.L., Horn, G.T., et al. Nature, 324, 163–166, 1986.PubMedCrossRefGoogle Scholar
  2. 2).
    Trucco, M., Rosenshine, S., Cascino, I., et al. Immunogenetics, 24, 184–190, 1986.PubMedGoogle Scholar
  3. 3).
    Carrington, M., Miller, T., White, M., et al. Hum. Genet. 33, 208–212, 1992.Google Scholar
  4. 4).
    Lo, Y.-M.D., Pater, P., Mehal, W.Z., et al. Nucl. Acids Res. 20, 1005–1009, 1992.PubMedCrossRefGoogle Scholar
  5. 5).
    Akiyama, K., Yoshii, T. and Ishiyama, I. The physico -chemical biology. (Japan). 37, 41–44, 1993.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Katsunori Akiyama
    • 1
  • Tomio Yoshii
    • 1
  • Ikuo Ishiyama
    • 1
  1. 1.Department of Forensic MedicineTeikyo University School of MedicineJapan

Personalised recommendations