Advertisement

Comparison of DNA-Extraction Methods from Compact Bone Tissue

  • K. Lalu
  • P. J. Karhunen
  • A. Sajantila
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

Identification of highly decomposed remains of a corpse can be accomplished by DNA-typing (1). In some cases the only source of DNA is sceletal bones. A simple and rapid DNA-extraction method for bone tissue would have use in routine casework as well as in working with a large number of mass disaster victims. In this work we have compared three methods to extract DNA from compact bone tissue.

Keywords

Allelic Ladder National Public Health Institute Boiling Method Minisatellite Locus Medicolegal Autopsy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Jeffreys AJ et al (1992) For Sci Int 56: 65–76Google Scholar
  2. 2.
    Maijer H et al (1992) Biochim Biophys Res Comm 183: 367–374CrossRefGoogle Scholar
  3. 3.
    Higuchi R (1989) In: Erlich (ed) PCR Technology-Principles and applications for DNA amplification 31–39Google Scholar
  4. 4.
    Sambrook et al (1989) Molecular Cloning-A Laboratory Manual 2nd edGoogle Scholar
  5. 5.
    Kasai K et al (1990) J For Sci 35: 1196–1200Google Scholar
  6. 6.
    Wolf RK et al (1988) Genomics 3: 347–351CrossRefGoogle Scholar
  7. 7.
    Kimpton C et al (1992) Hum Mol Genet 1: 287PubMedCrossRefGoogle Scholar
  8. 8.
    Edwards a et al (1991) Am J Hum Genet 49: 746–756Google Scholar
  9. 9.
    Budowle B et al (1991) Am J Hum Genet 48: 137–144PubMedGoogle Scholar
  10. 10.
    Allen RC et al (1989) Biotechniques 7: 736–744PubMedCrossRefGoogle Scholar
  11. 11.
    Sajantila A et al (1992) Biotechniques 12: 16–22Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • K. Lalu
    • 1
  • P. J. Karhunen
    • 2
  • A. Sajantila
    • 3
  1. 1.Department of Forensic MedicineUniversity of HelsinkisFinland
  2. 2.Departments of Forensic MedicineUniversity of Tampere and KuopioFinland
  3. 3.National Public Health InstituteHelsinkiFinland

Personalised recommendations