Abstract
The D1S80 locus is a VNTR region, whose moderate repetition (units of 16 bp repeated in tandem 15–40 times) allows its analysis after amplification by the polymerase chain reaction (Kasai et al. 1990) and its genotyping after fractionation of the resulting fragments in high resolution PAGE followed by silver staining (Budowle et al. 1991). The possibility of analyzing this locus by PCR makes it a useful tool for the analysis of forensic samples which provide degraded or very low quantities of DNA. Moreover, the D1580 locus displays a high number of different alleles in all the populations studied, and due to its high power of discrimination, this locus is becoming a widely used system in genetic identification testing. So, in order to develope a simple system of amplification of the D1S80 locus, we have developed a new protocol using a micro thermal cycler, its advantages consisting of a reduction in time and costs. On the other hand, a study of the allele distribution of the D1S80 locus in a sample of 150 individuals residing in the Basque Country has been carried out using our procedure. We can also see that this technique is applicable to DNA samples obtained by different extraction procedures (phenol-chloroform, Chelex resin, rapid digestion) as well as from different sources (blood, blood or semen stains, hair roots).
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© 1994 Springer-Verlag Berlin Heidelberg
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Fernández-Fernández, I. et al. (1994). Polymorphism of the D1S80 Locus in Basque Country Population and Its Forensic Applications. In: Bär, W., Fiori, A., Rossi, U. (eds) Advances in Forensic Haemogenetics. Advances in Forensic Haemogenetics, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78782-9_33
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DOI: https://doi.org/10.1007/978-3-642-78782-9_33
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-57643-3
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