HLA.DQ-Alpha and D1S80 Data from a French Caucasian Population Random Sample Versus a Sample of Aircrash Victims

  • H. Pfitzinger
  • B. Ludes
  • P. Mangin
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 5)

Abstract

On January 20, 1992, an airbus A320 crashed in Strasbourg area: among the 90 passengers and the 6 crew, 87 died and 9 survived. Identification of the victims was investigated using conventional methods (dental records, physical and X rays examinations) and DNA fingerprinting. Then, the complete reconstitution of most heavy mutilated bodies was performed by DNA typing at the HLA.DQ-Alpha (1) and D1S80 (2) loci for all the human fragments. The HLA.DQ-Alpha and D1S80 data collected for 84 and 85 victims respectively were compared to the data obtained for a sample of individuals taken at random from the french caucasian population. Allele and genotype frequencies at the HLA.DQ-Alpha and D1S80 loci were determined by polymerase chain reaction (PCR) amplification.

Keywords

Agarose Bromide Ethidium 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Saiki RK, Walsh PS, Lewenson CH, Herlich HA (1989) Genetic analysis of amplified DNA with immobilized sequence specific oligonucleotide probes. Proc Natl Acad Sci USA 86: 6230–6234.PubMedCrossRefGoogle Scholar
  2. 2).
    Budowle B, Chakraborty R, Giusti A, Eisenberg AJ and Allen RC (1990) Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE. Am J Hum Genet 48: 137–144.Google Scholar
  3. 3).
    Helmuth R, Fildes N, Blake E, Luce MC, Chimera J, Madij R, Gorodezky C, Stoneking M, Schmill N, Klitz W, Higuchi R and Erlich HA (1990) HLA.DQ-Alpha allele and genotype frequencies in various human populations, determined by using enzymatic amplification and oligonucleotide probes. Am J Hum Genet 47: 515–523.PubMedGoogle Scholar
  4. 4).
    Comey C and Budowle B (1991) Validation studies on the analysis of the HLA.DQ-Alpha locus using the polymerase chain reaction. J Forensic Sci 36: 1633–1648.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • H. Pfitzinger
    • 1
  • B. Ludes
    • 1
  • P. Mangin
    • 1
  1. 1.Institut de Médecine LégaleFaculté de MédecineStrasbourgFrance

Personalised recommendations