RNA polymerase III

Genes, factors and transcriptional specificity
  • Ian M. Willis
Part of the EJB Reviews book series (EJB REVIEWS, volume 1993)


Recent studies on RNA polymerase III (pol III) gene transcription have provided a new awareness of the molecular complexity of this process. Fortunately, while the number of transcription components has been increasing, fundamental similarities have emerged regarding the function of eukaryotic promoter elements and the factors that bind them to form preinitiation complexes. Among these, the ability of transcription factor IIIB (TFIIIB) and pol III to transcribe the Saccharomyces cerevisiae U6 gene suggests that the concept of a minimal pol II promoter comprising a TATA box and an initiator region has a parallel in the pol III system. Furthermore, for each of the three classes of eukaryotic RNA polymerase, the assembly of transcription preinitiation complexes and, to some extent, the nature of these complexes appears to be more similar than was previously anticipated.


tRNA Gene Promoter Structure Preinitiation Complex Negative Cofactor Yeast Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



transcription factor


TATA-binding protein


proximal sequence element


distal sequence element


Epstein-Barr virus-encoded RNA




activating transcription factor


negative cofactor


proximal element-binding protein


PSE transcription factor




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  1. Archambault, J., Milne, C. A. Schapper, K., Baum, B., Friesen, J. D. & Segall, J. (1992) J. Biol. Chem. 267, 3282–3288.PubMedGoogle Scholar
  2. Bartholomew, B., Kassavetis, G. A., Braun, B. R. & Geiduschek, E. R. (1990) EMBO J. 9, 2197–2205.PubMedGoogle Scholar
  3. Bartholomew, B., Kassavetis, G. A. & Geiduschek, E. R. (1991) Mol. Cell. Biol. 11, 5181–5189.PubMedGoogle Scholar
  4. Bartholomew, B., Kassavetis, G. A., Durkovich, D. & Geiduschek, E. R. (1992) J. Cell. Biochem. 16, 126.Google Scholar
  5. Bordonné, R. & Guthrie, C. (1992) Nucleic Acids Res. 20, 479–485.PubMedCrossRefGoogle Scholar
  6. Braun, B. R., Riggs, D. L., Kassavetis, G. A. & Geiduschek, E. R. (1989) Proc. Natl Acad. Sci. USA 86, 2530–2534.PubMedCrossRefGoogle Scholar
  7. Bredow, S., Kleinert, H. & Benecke, B.-J. (1989) Gene (Amst.) 86, 217–225.CrossRefGoogle Scholar
  8. Bredow, S., Surig, D., Muller, J., Kleinert, H. & Benecke, B.-J. (1990) Nucleic Acids Res. 18, 6779–6784.PubMedCrossRefGoogle Scholar
  9. Brow, D. A. & Guthrie, C. (1990) Genes & Dev. 4, 1345–1356.CrossRefGoogle Scholar
  10. Buratowski, S. & Zhou, H. (1992) Cell 71, 221–230.PubMedCrossRefGoogle Scholar
  11. Cohen, I. & Reynolds, W. F. (1991) Nucleic Acids Res. 19,4753–4759.PubMedCrossRefGoogle Scholar
  12. Carbon, R & Krol, A. (1991) EMBO J. 10, 2941–2948.Google Scholar
  13. Cornai, L., Tanese, N. & Tjian, R. (1992) Cell 68, 965–976.CrossRefGoogle Scholar
  14. Cormack, B. R. & Struhl, K. (1992) Cell 69, 685–696.PubMedCrossRefGoogle Scholar
  15. Cromlish, J. A. & Roeder, R. G. (1989) J. Biol. Chem. 264, 18100–18109.PubMedGoogle Scholar
  16. Frendewey, D., Barta, I., Gillespie, M. & Potashkin, J. (1990) Nucleic Acids Res. 18, 2025–2032.PubMedCrossRefGoogle Scholar
  17. Gabrielsen, O. S., Marzouki, N., Ruet, A., Sentenac, A. & Fromageot, P. (1989) J. Biol. Chem. 264, 7505–7511.PubMedGoogle Scholar
  18. Gabrielsen, O. S. & Sentenac, A. (1991) Trends Biochem. 16,412–416.CrossRefGoogle Scholar
  19. Geiduschek, E. R & Tocchini-Valentini, G. P. (1988) Annu. Rev. Biochem. 57, 873–914.PubMedCrossRefGoogle Scholar
  20. Gunderson, S. I., Knuth, M. W. & Burgess, R. R. (1990) Genes & Dev. 4, 2048–2060.CrossRefGoogle Scholar
  21. Gupta, S. & Reddy, R. (1991) Nucleic Acids Res. 19, 2073–2075.PubMedCrossRefGoogle Scholar
  22. Hallberg, E. M., Fung, R & Hallberg, R. L. (1992) Nucleic Acids Res. 20, 912.PubMedCrossRefGoogle Scholar
  23. Hann, B. C. & Walter, R (1991) Cell 67, 131–144.PubMedCrossRefGoogle Scholar
  24. Hannon, G. J., Chubb, A., Maroney, R. A., Hannon, G., Altman, S. & Nilsen, T. W. (1991) J. Biol. Chem. 266,22796–22799.PubMedGoogle Scholar
  25. Howe, J. G. & Shu, M.-D. (1989) Cell 57,825–834.PubMedCrossRefGoogle Scholar
  26. Huibregtse, J. M. & Engelke, D. R. (1989) Mol. Cell. Biol. 9, 3244–3252.PubMedGoogle Scholar
  27. Johnson, D. L. & Wilson, S. L. (1989) Mol. Cell. Biol. 9,2018–2024.PubMedGoogle Scholar
  28. Kassavetis, G. A., Riggs, D. L., Negri, R., Nguyen, L. H. & Geiduschek, E. P. (1989) Mol. Cell. Biol. 9, 2551–2566.PubMedGoogle Scholar
  29. Kassavetis, G. A., Braun, B. R., Nguyen, L. H. & Geiduschek, E. P. (1990) Cell 60,235–245.PubMedCrossRefGoogle Scholar
  30. Kassavetis, G. A., Bartholomew, B., Blanco, J. A., Johnson, T. E. & Geiduschek, E. R (1991) Proc. Natl Acad. Sci. USA 88, 7308–7312.PubMedCrossRefGoogle Scholar
  31. Klekamp, M. S. & Weil, P. A. (1986) J. Biol. Chem. 261,2819–2827.PubMedGoogle Scholar
  32. Kiss, T. & Filipowicz, W. (1992) Cell 70, 11–16.PubMedCrossRefGoogle Scholar
  33. Kiss, T., Marshallsay, C. & Filipowicz, W. (1991) Cell 65,517–526.PubMedCrossRefGoogle Scholar
  34. Knuth, M. W., Gunderson, S. I. & Burgess, R. R. (1990) J. Biol. Chem. 265, 17911–17920.PubMedGoogle Scholar
  35. Kovelman, R. & Roeder, R. G. (1992) J. Cell. Biochem. 16, 144.Google Scholar
  36. Kunkel, G. R. (1991) Biochim. Biophys. Acta 1088, 1–9.PubMedGoogle Scholar
  37. Lee, B. J., Kang, S. G. & Hatfield, D. (1989) J. Biol. Chem. 264, 9696–9702.PubMedGoogle Scholar
  38. Lee, J. Y., Evans, C. F. & Engelke, D. R. (1991a) Proc. Natl Acad. Sci. USA 88, 6986–6990.CrossRefGoogle Scholar
  39. Lee, D. K., Horikoshi, M. & Roeder, R. G. (1991b) Cell 67, 1241–1250.CrossRefGoogle Scholar
  40. Lescure, A., Carbon, P. & Krol, A. (1991) Nucleic Acids Res. 19, 435–441.PubMedCrossRefGoogle Scholar
  41. Léveillard, T., Kassavetis, G. A. & Geiduschek, E. R (1991) J. Biol. Chem. 266, 5162–5168.PubMedGoogle Scholar
  42. Lobo, S. & Hernandez, N. (1989) Cell 58, 55–67.PubMedCrossRefGoogle Scholar
  43. Lobo, S. M., Lister, J., Sullivan, M. L. & Hernandez, N. (1991) Genes & Dev. 5, 1477–1489.CrossRefGoogle Scholar
  44. López-de-León, A., Librizzi, M., Puglia, K. & Willis, I. M. (1992) Cell 71, 211–220.PubMedCrossRefGoogle Scholar
  45. Margottin, F., Dujardin, G., Gerard, M., Egly, J.-M., Huet, J. & Sentenac, A. (1991) Science 251,424–426.PubMedCrossRefGoogle Scholar
  46. Marzouki, N., Carnier, S., Ruet, A., Moenne, A. & Sentenac, A. (1986) Nature 323,176–178.PubMedCrossRefGoogle Scholar
  47. Mathews, M. B. & Shenk, T. (1991) J. Virol. 65,5657–5662.PubMedGoogle Scholar
  48. Mattaj, I. W., Dathan, N. A., Parry, H. D., Carbon, R. & Krol, A. (1988) Cell 55, 435–442.PubMedCrossRefGoogle Scholar
  49. Meisterernst, M. & Roeder, R. G. (1991) Cell 67, 557–567.PubMedCrossRefGoogle Scholar
  50. Meisterernst, M., Roy, A. L., Lieu, H. M. & Roeder, R. G. (1991) Cell 66, 981–993.PubMedCrossRefGoogle Scholar
  51. Moenne, A., Carnier, S., Anderson, G., Margottin, F., Beggs, J. & Sentenac, A. (1990) EMBO J. 9,271–277.PubMedGoogle Scholar
  52. Murphy, S., Yoon, J.-B., Gerster, T. & Roeder, R. G. (1992) Mol. Cell. Biol. 12, 3247–3261.PubMedGoogle Scholar
  53. Myslinski, E., Krol, A. & Carbon, P. (1992) Nucleic Acids Res. 20,203–209.PubMedCrossRefGoogle Scholar
  54. Ottonello, S., Rivier, D. H., Doolittle, G. M., Young, L. S. & Sprague, K. U. (1987) EMBO. 16, 1921–1927.Google Scholar
  55. Parsons, M. C. & Weil, P. A. (1990) J. Biol. Chem. 265,5095–5103.PubMedGoogle Scholar
  56. Parsons, M. C. & Weil, P. A. (1992) J. Biol. Chem. 267,2894–2901.PubMedGoogle Scholar
  57. Ptashne, M. (1988) Nature 335, 683–689.PubMedCrossRefGoogle Scholar
  58. Pugh, B. F. & Tjian, R. (1991) Genes & Dev. 5,1935–1945.CrossRefGoogle Scholar
  59. Reyes, V. M., Newman, A. & Abelson, J. (1986) Mol. Cell. Biol. 6,2436–2442.PubMedGoogle Scholar
  60. Rome, L., Kedersha, N. & Chugani, D. (1991) Trends Cell Biol. 1, 47–50.PubMedCrossRefGoogle Scholar
  61. Romero, D. P. & Blackburn, E. H. (1991) Cell 67, 343–353.PubMedCrossRefGoogle Scholar
  62. Ruet, A., Carnier, S., Smagowicz, W., Sentenac, A. & Fromageot, R. (1984) EMBO J. 3,343–350.PubMedGoogle Scholar
  63. Schneider, H. R., Waldschmidt, R. & Seifart, K. H. (1990) Nucleic Acids Res. 18, 4743–4750.PubMedCrossRefGoogle Scholar
  64. Schultz, R, Marzouki, N., Marck, C., Ruet, A., Oudet, R. & Sentenac, A. (1989) EMBO J. 8, 3815–3824.PubMedGoogle Scholar
  65. Schultz, M. C., Reeder, R. H. & Hahn, S. (1992) Cell 69, 697–702.PubMedCrossRefGoogle Scholar
  66. Seifart, K. H., Wang, L., Waldschmidt, R., Jahn, D. & Wingender, E. (1989) J. Biol. Chem. 264, 1702–1709.PubMedGoogle Scholar
  67. Sharp, S., Schaack, J., Cooley, L., Burke, D. J. & Soll, D. (1985) CRC Grit. Rev. Biochem. 19, 107–144.CrossRefGoogle Scholar
  68. Simmen, K. A., Bermes, J., Parry, H. D., Stunnenberg, H. G., Berkenstam, A., Cavallini, B., Egly, J.-M. & Mattaj, I. W. (1991) EMBO. 110, 1853–1862.Google Scholar
  69. Smith, D. R., Jackson, I. J. & Brown, D. D. (1984) Cell 37, 645–652.PubMedCrossRefGoogle Scholar
  70. Specht, T., Wolters, J. & Erdmann, V. A. (1991) Nucleic Acids Res. 19,2189–2191.PubMedGoogle Scholar
  71. Sprinzl, M., Dank, N., Nock, S. & Schön, A. (1991) Nucleic Acids Res. 19,2127–2171.PubMedGoogle Scholar
  72. Starr, D. B. & Hawley, D. K. (1991) Cell 67, 1231–1240.PubMedCrossRefGoogle Scholar
  73. Stillman, D. J., Better, M. & Geiduschek, E. R. (1985) J. Mol. Biol. 185, 451–455.PubMedCrossRefGoogle Scholar
  74. Sussman, D. J., Chung, J. & Leder, R. (1991) Nucleic Acids Res. 19, 5045–5052.PubMedCrossRefGoogle Scholar
  75. Swanson, R. N., Consea, C., Lefebvre, O., Caries, C., Ruet, A., Quemeneur, E., Gagnon, J. & Sentenac, A. (1991) Proc. Natl Acad. Sci. USA 88, 4887–4891.PubMedCrossRefGoogle Scholar
  76. Taneja, R., Gopalkrishnan, R. & Gopinathan, K. R (1992) Proc. Natl Acad. Sci. USA 89,1070–1074.PubMedCrossRefGoogle Scholar
  77. Topper, J. N., Bennett, J. L. & Clayton, D. A. (1992) Cell 70,1620.CrossRefGoogle Scholar
  78. Waldschmidt, R. & Seifart, K. H. (1992) J. Biol. Chem. 267,16359–16364.PubMedGoogle Scholar
  79. Waldschmidt, R., Wanandi, I. & Seifart, K. H. (1991) EMBO J. 10,2595–2603.Google Scholar
  80. Wang, C. K. & Weil, R. A. (1989) J. Biol. Cheng. 264,1092–1099.Google Scholar
  81. White, R. J., Jackson, S. P. & Rigby, R. W. J. (1992) Proc. Natl Acad. Sci. USA 89,1949–1953.PubMedCrossRefGoogle Scholar
  82. Willis, I., Hottinger, H., Pearson, D., Chisholm, V., Leupold, U. & Still, D. (1984) EMBO J. 3, 1573–1580.PubMedGoogle Scholar
  83. Willis, I. M., Schmidt, R. & Söll, D. (1989) EMBO J. 8, 4281–4288.PubMedGoogle Scholar
  84. Willis, I. M., Oksman, A. & López-de-León, A. (1992) Nucleic Acids Res. 20, 3725–3730.PubMedCrossRefGoogle Scholar
  85. Wolfe, A. P. & Brown, D. D. (1988) Science 241, 1626–1632. Wolin, S. L. (1985) Trends Genet. 1, 201–204.Google Scholar
  86. Woychik, N. A. & Young, R. A. (1992) Proc. Natl Acad. Sci. USA 89, 3999–4003.PubMedCrossRefGoogle Scholar
  87. Yoshinaga, S. K., Boulanger, R. A. & Berk, A. J. (1987) Proc. Natl Acad. Sci. USA 84, 3585–3589.PubMedCrossRefGoogle Scholar
  88. Yoshinaga, S. K., L’Etoile, N. & Berk, A. J. (1989) J. Biol. Chem. 264,10726–10731.PubMedGoogle Scholar
  89. Young, L. S., Dunstan, H. M., Witte, R. R., Smith, T. R, Ottonello, S. & Sprague, K. U. (1991) Science 252,542–546.PubMedCrossRefGoogle Scholar
  90. Yu, G.-L., Bradley, J. D., Attardi, L. D. & Blackburn, E. H. (1990) Nature 344,126–132.PubMedCrossRefGoogle Scholar

Copyright information

© Federation of European Biochemical Societies 1994

Authors and Affiliations

  • Ian M. Willis
    • 1
  1. 1.Department of Biochemistry, Albert Einstein College of MedicineJack and Pearl Resnick CampusBronxUSA

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