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Contemporary Aspects of Radioimmunoassay Development for Drug Analysis

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Pharmacokinetics of Drugs

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 110))

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Abstract

The application of radioimmunoassay (RIA) for quantitation of pharmaceutical drug concentration in biological matrices has increased in recent years due to several factors. The first reason has been the development of more efficacious drugs, resulting in lower effective doses and subsequent lower concentrations in matrices such as plasma or urine. Although traditional chromatographic analytical procedures such as high-performance liquid chromatography (HPLC) or gas chromatography (GC) have demonstrated improved sensitivity, compounds continue to be developed that cannot be measured by HPLC or GC. A second factor involved in the increased use of RIA is an enhanced awareness and willingness to develop the complex synthetic chemistry necessary to produce the requisite immunological and radiochemical reagents for RIA. Lastly, with the advent of radioactivity counters that can rapidly count large numbers of assay samples and perform the required data reduction, RIA has become more time efficient than chromatographic methods.

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Authors
  1. G. D. Nordblom
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  2. C. M. Barksdale
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Editor information

Editors and Affiliations

  1. Pharmacokinetics/Drug Metabolism Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, 2800 Plymouth Road, 48105-1047, Ann Arbor, MI, USA

    Peter G. Welling Ph.D., D.Sc. (Vice President) (Vice President)

  2. Clinical Research Unit, Department of Psychiatry, 47, Rue du XXXI Décembre, CH-1207, Geneva, Switzerland

    Luc P. Balant Ph.D. (Reader) (Reader)

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© 1994 Springer-Verlag Berlin Heidelberg

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Nordblom, G.D., Barksdale, C.M. (1994). Contemporary Aspects of Radioimmunoassay Development for Drug Analysis. In: Welling, P.G., Balant, L.P. (eds) Pharmacokinetics of Drugs. Handbook of Experimental Pharmacology, vol 110. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78680-8_2

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  • DOI: https://doi.org/10.1007/978-3-642-78680-8_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78682-2

  • Online ISBN: 978-3-642-78680-8

  • eBook Packages: Springer Book Archive

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