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The β Sliding Clamp of E. coli DNA Polymerase III Holoenzyme Balances Opposing Functions

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Nucleic Acids and Molecular Biology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 8))

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Abstract

The β subunit of E. coli DNA polymerase III holoenzyme (pol III holoenzyme) anchors this multiprotein chromosomal replicase to DNA for fast and highly processive replication (Kornberg and Baker 1991; Stukenberg et al. 1991). Its ring shape (Fig. 1) allows it to completely encircle DNA while freely diffusing along the duplex (Stukenberg et al. 1991; Kong et al. 1992). The β “sliding clamp” confers onto pol III holoenzyme a high degree of processivity (50kb; Fay et al. 1981) and a rapid speed of synthesis (750 nucleotides/s; O’Donnell and Kornberg 1985) which results from the continual proximity of the polymerase and DNA through their mutual association with β (Stukenberg et al. 1991).

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Authors and Affiliations

  1. Microbiology Department, Hearst Research Center, Howard Hughes Medical Institute, Cornell Medical College, New York, NY, 10021, USA

    M. O’donnell, P. T. Stukenberg, R. Onrust & N. Yao

  2. Laboratories of Molecular Biophysics, Howard Hughes Medical Institute, The Rockefeller University, New York, NY, 10021, USA

    J. Kuriyan & X.-P. Kong

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  1. M. O’donnell
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  2. J. Kuriyan
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  3. X.-P. Kong
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  4. P. T. Stukenberg
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  5. R. Onrust
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  6. N. Yao
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Editors and Affiliations

  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, Dundee, DD1 4HN, UK

    David M. J. Lilley

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© 1994 Springer-Verlag Berlin Heidelberg

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O’donnell, M., Kuriyan, J., Kong, XP., Stukenberg, P.T., Onrust, R., Yao, N. (1994). The β Sliding Clamp of E. coli DNA Polymerase III Holoenzyme Balances Opposing Functions. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78666-2_11

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  • DOI: https://doi.org/10.1007/978-3-642-78666-2_11

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