Skip to main content

Cryopreservation of Protoplast, Suspension and Callus Cultures

  • Chapter
Genetic Preservation of Plant Cells in Vitro

Part of the book series: Springer Lab Manuals ((SLM))

Abstract

This chapter deals with cryopreservation of the simplest types of in vitro culture, at least in terms of differentiation and organisation, and also contains general information concerning aspects of cryopreservation which are relevant to other subjects in this volume, which will be cross-referenced elsewhere.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bajaj YPS (1983) Survival of somatic hybrid protoplasts of wheat X pea and rice X pea subjected to -196 °C. Indian J Exp Biol 21:120–122

    Google Scholar 

  • Bajaj YPS (1988) Regeneration of plants from frozen (—196°C) protoplasts of Atropa belladona L., Datura innoxia Mill, and Nicotiana tabacum L. Indian J Exp Biol 26:289–292

    Google Scholar 

  • Bajaj YPS (1989) Cryopreservation of plant protoplasts. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 8: Plant protoplasts and genetic engineering. Springer, Berlin Heidelberg New York, pp 97–106

    Chapter  Google Scholar 

  • Cellarova E, Cernicka T, Vranova E, Brutovska R, Lapar M (1992) Viability of Chamomilla recutita L. cells after cryopreservation. Cryo Lett 13:37–42

    Google Scholar 

  • Cocking EC (1972) Plant cell protoplasts — isolation and development. Annu Rev Plant Physiol 23:29–50

    Article  CAS  Google Scholar 

  • Dodds JH (1991) In vitro methods for conservation of plant genetic resources. Chapman & Hall, London

    Book  Google Scholar 

  • Dussert S, Mauro MC, Deloire A, Hamon S, Engelmann F (1991) Cryopreservation of grape embryogenic cell suspensions 1. Influence of pretreatment, freezing and thawing conditions. Cryo Lett 12:287–298

    Google Scholar 

  • Dussert S, Mauro MC, Engelmann F (1992) Cryopreservation of grape embryogenic cell suspensions 2. Influence of post-thaw culture conditions and application to different strains. Cryo Lett 13:15–22

    Google Scholar 

  • Engelmann F (1992) Effect of freezing in liquid nitrogen on the properties of a soybean (Glycine max L. var. acme) callus strain used as a bioassay for cytokinin activity. Cryo Lett 13:331–336

    Google Scholar 

  • Finkle BJ, Ulrich JM, Schaeffer GW, Sharpe F Jr (1983) Cryopreservation of rice cells. In: Inst of Genetics Academis Sinica/Int Rice Res Inst (eds) Cell and tissue culture techniques for cereal crop improvement. Gordon & Breach, New York, pp 343–369

    Google Scholar 

  • Gazeau CM, Blanchon C, Dereuddre J (1992) Freeze-preservation of Catharanthus protoplasts at liquid nitrogen temperature. Comparison with cells. Cryo Lett 13:149–158

    Google Scholar 

  • Gnamapragasam S, Vasil K (1990) Plant regeneration from a cryopreserveed embryogenic cell suspension of a commercial sugarcane hybrid (Saccharum sp.). Plant Cell Rep 9:419–423

    Google Scholar 

  • Grout BWW, Morris GJ (1987) The effects of low temperatures on biological systems. Edward Arnold, London

    Google Scholar 

  • Heslop-Harrison J, Heslop-Harrison Y (1977) Evaluation of pollen viability by enzymatically induced fluorescence: intracellular hydrolysis of fluorescein diacetate. Stain Technol 45:115–121

    Google Scholar 

  • Kartha KK (1982) Cryopreservation of periwinkle, Catharanthus roseus, cells cultured in vitro. Plant Cell Rep 1:135–138

    Article  Google Scholar 

  • Kartha K (ed) (1985) Cryopreservation of plant cells and organs. CRC Press, Boca Raton

    Google Scholar 

  • Kuriyama A, Watanabe K, Ueno S, Mitsuda H (1990) Effect of post-thaw treatment on the viability of cryopreserved Lavandula vera cells. Cryo Lett 11:171–178

    Google Scholar 

  • Nishizawa S, Sakai A, Amano Y, Matsuzawa T (1992) Cryopreservation of asparagus (Asparagus officinalis) embryogenic cells and subsequent plant regeneration by a simple freezing method. Cryo Lett 13:379–388

    Google Scholar 

  • Panis BJ, Withers LA, De Langhe EAL (1990) Cryopreservation of Musa suspension culutres and subsequent regeneration of plants. Cryo Lett 11:337–350

    Google Scholar 

  • Power JB, Davey M (1990) Protoplasts of higher and lower plants. In: Pollard JW, Walker JM (eds) Methods in molecular biology, vol 6: Plant cell and tissue culture. Humana Press, New Jersey, pp 237–259

    Chapter  Google Scholar 

  • Pritchard HW, Grout BWW, Short KC (1986) Osmotic stress as a pregrowth procedure for cryopreservation 3. Cryobiology of sycamore and soybean cell suspensions. Ann Bot 57:379–388

    Google Scholar 

  • Steponkus PL (1985) Cryobiology of isolated protoplasts — applications to plant cell cryopreservation. In: Karhta K (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, pp 49–60

    Google Scholar 

  • Steponkus PL, Lamphear FO (1967) Refinement of the triphenyl tetrazolium chloride method of determining cold injury. Plant Physiol 42:1423–1426

    Article  PubMed  CAS  Google Scholar 

  • Takeuchi M, Matushima H, Sugawara Y (1982) Totipotency and viability of protoplasts after long-term freeze preservation. In: Fujiwara A (ed) Plant tissue culture 1982. Japanese Association for Plant Tissue Culture, Tokyo, pp 797–798

    Google Scholar 

  • Widholme JM (1972) The use of fluorescein diacetate for determining viability of cultured plant cells. Stain Technol 47:189–194

    Google Scholar 

  • Withers LA (1980) Preservation of germplasm. In: Vasil IK (ed) International review of cytology. Supplement 11B Perspectives in plant cell and tissue culture. Academic Press, New York, pp 101–136

    Google Scholar 

  • Withers LA (1985a) Cryopreservation of cultured plant cells and protoplasts. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, pp 244–267

    Google Scholar 

  • Withers LA (1985b) Cryopreservation and storage of germplasm. In: Dixon RA (ed) Plant cell cultures — a practical approach. IRL Press, Oxford, pp 169–192

    Google Scholar 

  • Withers LA (1987) The low-temperature preservation of plant cell, tissue and organ cultures and seed for genetic conservation and improved agricultural practice. In: Grout BWW, Morris JG (eds) The effects of low temperatures on biological systems. Edward Arnold, London, pp 389–409

    Google Scholar 

  • Withers LA (1990) Cryopreservation of plant cells. In: Pollard JW, Walker JM (eds) Methods in molecular biology, vol 6. Plant cell and tissue culture. The Humana Press, New Jersey, pp 39–48

    Chapter  Google Scholar 

  • Withers LA, King PJ (1980) A simple freezing unit and cryopreservation method for plant cell suspensions. Cryo Lett 1:213–220

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer- Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Grout, B.W.W. (1995). Cryopreservation of Protoplast, Suspension and Callus Cultures. In: Grout, B. (eds) Genetic Preservation of Plant Cells in Vitro. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78661-7_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-78661-7_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-48968-6

  • Online ISBN: 978-3-642-78661-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics