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Minimal Growth Storage

  • Chapter
Genetic Preservation of Plant Cells in Vitro

Part of the book series: Springer Lab Manuals ((SLM))

Abstract

Minimal growth storage is the most direct way of restricting growth and development of in vitro materials and is most usually applied to differentiated plantlets and developing meristem cultures. It may also be applied to larger callus masses. At its simplest, lowering the environmental temperature of cultures below their active growing temperature is used to significantly extend the interval between subcultures. Care must be taken to avoid temperatures below freezing or levels where other temperature-related injuries might occur, e.g. chilling injury (Lyons et al. 1979; Wilson 1987). Despite the genetic stability inherent in organised plant structures, such as mer-istems, there are risks to DNA structure, integrated metabolism and viability under conditions of slow growth such as resulting from the oxidative activity of free radicals (Benson 1990). Non-lethal effects can also result from this type of damage and should be screened for by systematic evaluation of aspects of structure and function before, and after, storage (Potter and Jones 1991; Withers 1991). Minimal growth storage provides a relatively low level of stability and protection for in vitro subjects, as increase in biomass, development and metabolism are slowed rather than halted. However, the technique has the benefits of being inexpensive and requiring little, if anything, in the way of specialised equipment or expertise.

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Authors
  1. B. W. W. Grout
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Editors and Affiliations

  1. Crisp Innovar Ltd., Glebe House, Station Road, NR 110 4NB, Reepham, Norfolk, UK

    Brian Grout

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© 1995 Springer- Verlag Berlin Heidelberg

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Grout, B.W.W. (1995). Minimal Growth Storage. In: Grout, B. (eds) Genetic Preservation of Plant Cells in Vitro. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78661-7_2

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  • DOI: https://doi.org/10.1007/978-3-642-78661-7_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-48968-6

  • Online ISBN: 978-3-642-78661-7

  • eBook Packages: Springer Book Archive

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