Glycosaminoglycan Distribution Pattern in Dupuytren’s Contracture Biopsies

  • N. Gässler
Conference paper

Abstract

Glycosaminoglycans consist of polysaccharide chains attached to a core protein. Together with the core protein they make up the proteoglycans. To a large extent the structure of the glycosaminoglycans was established by the work of Meyer (1970). Glycosaminoglycans are linear polymers of repeated disaccharides. The number of repeated disaccharides varies, but typical values are in the order of 50. The constituent monosaccharide residues usually show the chair C-1 conformation. The reason this conformation is favored by the D-monosaccharides lies within the position of the substituents. In the C-1 conformation, most will occupy equatorial positions and maintain the largest possible distance from one another. The length of the disaccharide unit, as measured by X-ray crystallography, varies from 0.93 to 0.97 nm for the different glycosaminoglycans (Heinegard and Paulson 1984).

Keywords

High Performance Liquid Chromatography Enzymatic Degradation Oligosaccharide Galactose Monosaccharide 

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References

  1. Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem 4: 330–334PubMedCrossRefGoogle Scholar
  2. Farndale RW, Buttle DJ, Barrett AJ (1986) Improved quantitation and discrimination of sulfated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 883: 173–177PubMedGoogle Scholar
  3. Flint MH, Gillard GC, Reilly HC (1982) The glycosaminoglycans of Dupuytren’s disease. Connect Tissue Res 9: 173–179PubMedCrossRefGoogle Scholar
  4. Gurr E, Pallasch G, Tunn S, Tamm C, Delbriick A (1985) High performance liquid chromatographic assay of disaccharides and oligosaccharides produced by the digestion of glycosaminoglycans with chondroitin sulphate lyases. J Clin Chem Clin Biochem 23: 77–87PubMedGoogle Scholar
  5. Heinegard D, Paulson M (1984) Structure and metabolism of proteoglycans. In: Piez KA, Reddi AH (eds) Extracellular matrix biochemistry. Elsevier, New York, pp 277–328Google Scholar
  6. Meyer K (1970) Reflections on “mucopolysaccharides” and their protein polysaccharide complexes. In: Balazs EA (ed) The Chemistry and molecular biology of the intracellular matrix, vol 1. Academic, London, pp 5–24Google Scholar
  7. Scott JE, Haigh M, Nusgens B, Lapiere CM (1989) Proteoglycan: collagen interactions in dermatosporatic skin and tendon. An electron histochemical study using cupromeronic blue in a critical electrolyte concentration method. Matrix 9: 437–442Google Scholar
  8. Tunn S, Gurr E, Delbriick A, Buhr T, Flory J (1988) The distribution of unsulphated and sulphated glycosaminoglycans in palmar fascia from patients with Dupuytren’s disease and healthy subjects. J Clin Chem Clin Biochem 26: 7–14PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • N. Gässler

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