Glycosaminoglycan Distribution Pattern in Dupuytren’s Contracture Biopsies
Glycosaminoglycans consist of polysaccharide chains attached to a core protein. Together with the core protein they make up the proteoglycans. To a large extent the structure of the glycosaminoglycans was established by the work of Meyer (1970). Glycosaminoglycans are linear polymers of repeated disaccharides. The number of repeated disaccharides varies, but typical values are in the order of 50. The constituent monosaccharide residues usually show the chair C-1 conformation. The reason this conformation is favored by the D-monosaccharides lies within the position of the substituents. In the C-1 conformation, most will occupy equatorial positions and maintain the largest possible distance from one another. The length of the disaccharide unit, as measured by X-ray crystallography, varies from 0.93 to 0.97 nm for the different glycosaminoglycans (Heinegard and Paulson 1984).
KeywordsHigh Performance Liquid Chromatography Enzymatic Degradation Oligosaccharide Galactose Monosaccharide
Unable to display preview. Download preview PDF.
- Heinegard D, Paulson M (1984) Structure and metabolism of proteoglycans. In: Piez KA, Reddi AH (eds) Extracellular matrix biochemistry. Elsevier, New York, pp 277–328Google Scholar
- Meyer K (1970) Reflections on “mucopolysaccharides” and their protein polysaccharide complexes. In: Balazs EA (ed) The Chemistry and molecular biology of the intracellular matrix, vol 1. Academic, London, pp 5–24Google Scholar
- Scott JE, Haigh M, Nusgens B, Lapiere CM (1989) Proteoglycan: collagen interactions in dermatosporatic skin and tendon. An electron histochemical study using cupromeronic blue in a critical electrolyte concentration method. Matrix 9: 437–442Google Scholar