Interphase Cytogenetics of Childhood Acute Lymphoblastic Leukemia with Fluorescence In Situ Hybridization Using a Panel of Eight Chromosome-Specific DNA Probes
Fluorescence in situ hybridization (FISH) analysis, as introduced by Cremer et al. , is a useful technique for detecting numerical as well as certain structural chromosome aberrations in interphase nuclei [2,3]. Numerical chromosome anomalies are determined by enumerating the hybridization signals of specific repetitive α-satellite DNA probes. Thus, FISH which is also referred to as “interphase cytogenetics” extends the possibilities to evaluate numerical anomalies particularly in neoplasms, for example acute lymphoblastic leukemias (ALL), in which conventional cytogenetic analysis is hampered by a small number of metaphases and poor chromosome quality.
KeywordsFormaldehyde Leukemia Citrate Iodide Dextran
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