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Transendothelial Migration

  • Chapter
Adhesion in Leukocyte Homing and Differentiation

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 184))

Abstract

Important insights into the mechanisms of transendothelial migration of neutrophils have been gained through several technical approaches in vitro (Smith 1992). Chemotactic gradients across confluent endothelial cell (EC) monolayers in vitro promote transmigration of neutrophils (Taylor et al. 1981; Furie et al. 1984). Rarely >40% of the neutrophils contacting the monolayer migrate (Furie etal. 1984). Stimulation of confluent EC monolayers in vitro for 3h with interleukin-1 β (IL-1β), tumor necrosis factor-α (TNF-α) or endotoxin (LPS) also promotes rapid transmigration of previously unstimulated neutrophils (Smith etal. 1988). This occurs with EC monolayers grown on polycarbonate filters (Moser et al. 1989; Kuijpers etal. 1992a), human amniotic membrane (Furie and McHugh 1989) or type I collagen gels (Huber etal. 1991; Luscinskas etal. 1991). Cytokine-stimulated ECs apparently produce all of the factors necessary to induce transendothelial migration of not only neutrophils, but eosinophils (Ebisawa et al. 1992), monocytes (Hakkert et al. 1991), and subsets of T cells (Oppenheimer-Marks etal. 1990; Van Epps etal. 1989). When observed directly under phase contrast optics, spherical neutrophils settling onto previously activated EC monolayers are seen to become motile within 1–2min after contacting the apical surface of the monolayer (Smith etal. 1988; Luscinskas etal. 1991), and within 5–10min a high percentage (50%–90%) migrate beneath the monolayer.

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  1. Speros Martel Section of Leukocyte Biology, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, 77030-2399, USA

    C. W. Smith

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  1. C. W. Smith
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Editors and Affiliations

  1. Basel Institute for Immunology, Grenzacherstr. 487, 4005, Basel, Switzerland

    Dominique Dunon Ph.D. , Charles R. Mackay Ph.D.  & Beat A. Imhof Ph.D. ,  & 

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© 1993 Springer-Verlag Berlin · Heidelberg

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Smith, C.W. (1993). Transendothelial Migration. In: Dunon, D., Mackay, C.R., Imhof, B.A. (eds) Adhesion in Leukocyte Homing and Differentiation. Current Topics in Microbiology and Immunology, vol 184. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78253-4_16

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  • DOI: https://doi.org/10.1007/978-3-642-78253-4_16

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  • Print ISBN: 978-3-642-78255-8

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