Transendothelial Migration

  • C. W. Smith
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 184)


Important insights into the mechanisms of transendothelial migration of neutrophils have been gained through several technical approaches in vitro (Smith 1992). Chemotactic gradients across confluent endothelial cell (EC) monolayers in vitro promote transmigration of neutrophils (Taylor et al. 1981; Furie et al. 1984). Rarely >40% of the neutrophils contacting the monolayer migrate (Furie etal. 1984). Stimulation of confluent EC monolayers in vitro for 3h with interleukin-1 β (IL-1β), tumor necrosis factor-α (TNF-α) or endotoxin (LPS) also promotes rapid transmigration of previously unstimulated neutrophils (Smith etal. 1988). This occurs with EC monolayers grown on polycarbonate filters (Moser et al. 1989; Kuijpers etal. 1992a), human amniotic membrane (Furie and McHugh 1989) or type I collagen gels (Huber etal. 1991; Luscinskas etal. 1991). Cytokine-stimulated ECs apparently produce all of the factors necessary to induce transendothelial migration of not only neutrophils, but eosinophils (Ebisawa et al. 1992), monocytes (Hakkert et al. 1991), and subsets of T cells (Oppenheimer-Marks etal. 1990; Van Epps etal. 1989). When observed directly under phase contrast optics, spherical neutrophils settling onto previously activated EC monolayers are seen to become motile within 1–2min after contacting the apical surface of the monolayer (Smith etal. 1988; Luscinskas etal. 1991), and within 5–10min a high percentage (50%–90%) migrate beneath the monolayer.


Transendothelial Migration Neutrophil Adhesion Human Amniotic Membrane Leukocyte Adhesion Deficiency Chemotactic Gradient 
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Copyright information

© Springer-Verlag Berlin · Heidelberg 1993

Authors and Affiliations

  • C. W. Smith
    • 1
  1. 1.Speros Martel Section of Leukocyte Biology, Department of PediatricsBaylor College of MedicineHoustonUSA

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