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Determination of Phosphoinositides and Inositol Phosphates. Principles and Rules

  • G. W. Mayr
Conference paper
Part of the NATO ASI Series book series (volume 76)

Abstract

Only cellular PLC# activity can be determined presently without a HPLC or LC instrumentation. However, a state-of-art analysis of the complex metabolism of inositol phosphates and phosphoinositides requires sophisticated sample workup and HPLC analysis protocols. These are laborious and still mostly based on radioactive prelabeling of cells or tissues. The novel HPLC-MDD technique allows a fully automatized non-radioactive analysis (i) of masses of all inositol phosphates and polyphosphoinositides from any biological source and (ii) of binding or interconversion of such compounds by proteins in vitro. This and other frequently used techniques are compared.

Keywords

Phytic Acid Inositol Phosphate Sugar Phosphate Inositol Trisphosphate Radioligand Binding Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

MDD

metal-dye detection

PLC

phospholipase C/ phosphoinositidase C

PI3-kinase

phosphoinositide 3-kmase

PAR

4-(2-pyridylazo)resorcinol

HPLC

high performance liquid chromatography

LC

liquid chromatography

TLC

thin layer chromatography

HPTLC

high performance thin layer chromatography

TCA

trichloroacetic acid

DVB

divinylbenzene

PCA

perchloric acid

TCA

trichloroacetic acid: All D-myo-inositol phosphates and corresponding myo-inositol phospholipids (phosphoinositides) as well as glycerophospho-myo-inositol phosphates are abbreviated according to the 1988 relaxed NC-IUB recommendations e.g: Ins(1,4,5)P3, D-myo-inositol 1,4,5-trisphosphate; PtdIns(4,5)P2, phosphatidyl(1)-D-myo-inositol 4,5-bisphosphate; InsP4-(PP)2, bisdiphosphoinositol tetrakisphosphate; GroPIns(4,5)P2, α-glycerophospho(1)-D-myo-inositol 4,5-bisphosphate

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • G. W. Mayr
    • 1
  1. 1.Institut für Physiologische ChemieRuhr-Universität BochumBochum 1Germany

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