Helicobacter pylori Antigenic Preparations
The ultimate goal of any serodiagnostic assay must be 100% sensitivity and specificity although this is not realistic. To date, commercially available tests reproducibly give figures of greater than 90%. Increased specificity may be achieved by removing cross-reacting proteins from a pool of H. pylori antigenic material using a variety of purification techniques. However, as antigens are increasingly purified, a decrease in sensitivity may be observed. When fast protein hquid chromatography (FPLC) purified fractions of H. pylori were used as serodiagnostic antigens for a group of 45 patients (22 H. pylori positive and 23 H. pylori negative patients as determined by culture and [14C] urea breath test) a decrease in sensitivity was observed . However, the sensitivity was restored to 100% when selected fractions were recombined in an pooled antigen (Table 1).
KeywordsPylorus Infection Fast Protein Liquid Chromatography Urea Breath Test ELISA Antigen Pylorus Negative Patient
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