Advertisement

Colorimetric Evaluation of the Human Skin Blanching Assay

  • C. Queille-Roussel
  • L. Duteil
  • J. Czernielewski
  • H. Schaefer

Abstract

Normal skin color changes occur as consequences of either vascular or pigmentary modifications. Up to now, skin color modifications were usually assessed using various arbitrary visual scales. Numerous attempts to objectively quantity such modifications were performed. Thus, erythema caused by dermal vasodilation can be evaluated by either laser Doppler velocimetry [1] or spectrophotometry [2] However, vasoconstriction-induced blanching and disturbances of the melanin system (hypermelanosis, e. g., tanning, and hypo-, or amelanosis, e. g., nevus depigmentosa, vitiligo,) have remained difficult to quantity objectively.

Keywords

Visual Score Clobetasol Propionate Base Cream Betamethasone Dipropionate Untreated Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Willis CM, Stephen CJ, Stephen M, Wilkinson JD (1988) Assessment of erythema in irritant contact dermatitis. Comparison between visual scoring and laser Doppler flowmetry. Contact Dermatitis 18: 138–142Google Scholar
  2. 2.
    Mendelow AY, Forsyth A, Feather JW, Baillie AJ, Florence AT (1986) Skin reflectance measurements of patch test responses. Contact Dermatitis 15: 73–78PubMedCrossRefGoogle Scholar
  3. 3.
    McKenzie AW, Stoughton RB (1962) Method for comparing percutaneous absorption of steroids. Arch Dermatol 86: 608–610Google Scholar
  4. 4.
    Cornell RC, Stoughton RB (1985) Correlation of the vasoconstriction assay and clinical activity in psoriasis. Arch Dermatol 121: 63–67PubMedCrossRefGoogle Scholar
  5. 5.
    1983) Dermocorticoïdes (fiche de transparence). Direction de la Pharmacie et du Médica-ment, p, 13Google Scholar
  6. 6.
    Aiache JM, Lafaye C, Bouzat J, Rabier R (1980) Evaluation de la disponibilité topique des corticoïdes par thermographic. J Pharm Belg 35: 187–195PubMedGoogle Scholar
  7. 7.
    Thune P, The effect of corticosteroids on skin pulse. In Wilson IC, Marks R (eds) Mechanisms of topical corticosteroid activity. Churchill Livingstone, Edinburgh, pp 25–31Google Scholar
  8. 8.
    Altmeyer P, Zaun H (1976) Ergebnisse reflexphotometrischer Bestimmungen der Vasocon-striction nach topischer Applikation: IV. Zeitlicher Ablauf der Vasoconstriction und reaktiven Vasodilatation. Arch Dermatol Res 250: 419–420Google Scholar
  9. 9.
    Feather JW, Ryatt KS, Dawson JB et al. (1982) Reflectance spectrophotometric quantification of skin colour changes induced by topical corticosteroid preparations. Br J Dermatol 106: 436–444CrossRefGoogle Scholar
  10. 10.
    Leveque JL, Poelman MC, Legall F, De Rigal J (1985) New experimental approach to measure the skin-reflected light. Application to cutaneous erythema and blanching. Dermatologica 170: 12–16PubMedCrossRefGoogle Scholar
  11. 11.
    Kiraly K,Soos G (1976) Objective measurement of topiclaly applied corticosteroids. Der-matologica 152 [Suppl 1]: 133–137PubMedCrossRefGoogle Scholar
  12. 12.
    Seitz JC, Whitmore CG (1988) Measurement of erythema and tanning responses in human skin using a tri-stimulus colorimeter. Dermatologica 177: 70–75PubMedCrossRefGoogle Scholar
  13. 13.
    Babulak SW, Rhein LD, Scala DD et al. (1986) Quantification of erythema in soap chamber test using the Minolta Chroma (Reflectance) Meter: comparison of instrumental results with visual assessments. J Soc Cosmet Chem 37: 475–476Google Scholar
  14. 14.
    Westerhof W, Van Hasselt B, Kammeijer A (1986) Quantification of UV-induced erythema with a portable computer controlled chromameter. Photodermatology: 3: 310–314PubMedGoogle Scholar
  15. 15.
    Queille-Roussel C, Poncet M, Schaeffer H (1991) Quantification of skin colour changes induced by topical corticosteroid preparations using the Minolta Chroma Meter. B J Dermatol 124: 264–270CrossRefGoogle Scholar
  16. 16.
    Waring MJ, Monger L, Hollingsbee DA, Martin GP, Marriott C (1990) Evaluation of the Minolta Chromameter CR200 for the assessment of steroid induced blanching. Pharm Res 7/9 [Suppl]: S50Google Scholar
  17. 17.
    Pershing LK, Lambert LD, Krueger GG, Shah VP (1991) Reproducibility and correlation of the Minolta R chromameter, tape stripping and visual skin blanching in the assessment of bio-availability of topical 0.05% betamethasone dipropionate formulations. In: Scott RC, Guy RH, Hadgraft J, Bodde HE (eds) Prediction of percutaneous penetration: methods, measurements, modelling. IBC Technical servicesGoogle Scholar
  18. 18.
    Dupuis D, Rougier A, Roguet R, Lotte C, Kalopissis G (1984) In vivo relationship between horny layer reservoir effect and percutaneous absorption in human and rat. J Invest Dermatol 82: 353–356PubMedCrossRefGoogle Scholar
  19. 19.
    Chan SY, Li Wan Po A Quantitative skin blanching assay of corticosteroid creams using tri- stimulus colour analysis. J Pharm Pharmacol 44: 371–378Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • C. Queille-Roussel
    • 1
  • L. Duteil
    • 1
  • J. Czernielewski
    • 1
  • H. Schaefer
    • 2
  1. 1.Centre International de Recherches Dermatoloques Galderma (CIRD Galderma)ValbonneFrance
  2. 2.Centre de Pharmacologie Clinique Appliquée a la Dermatologie (CPCAD)Hôpital PasteurNiceFrance

Personalised recommendations