Transepidernial Water Loss Measurement by Means of an Evaporimeter

  • R. A. Tupker
  • J. Pinnagoda
  • P.-J. Coenraads
  • J. P. Nater


The skin is the interface of an organism with its environment, and therefore the basic role of the skin is protection, e. g., against water loss and entrance of external factors, such as irritants. The location of the permeation barrier depends on the physico-chemical characteristics of the substance concerned. Blank has noticed that the water diffusion rate was dependent on the number of stratum corneum layers stripped [5]. Based on this observation, the stratum corneum is considered to provide a uniformly impermeable layer which constitutes the main barrier against diffusional water loss [6]. The epidermal layers underneath the stratum corneum (i. e., viable epidermis) have a predominantly hydrophilic nature; water-soluble substances can be transported easily via the intercellular fluids. Lipophilic substances tend to accumulate and/or bind in the stratum corneum layers [25]. For the majority of compounds, therefore, the stratum corneum is the main penetration barrier [34].


Atopic Dermatitis Stratum Corneum Contact Dermatitis Evaporative Water Loss Transepidermal Water Loss 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • R. A. Tupker
  • J. Pinnagoda
  • P.-J. Coenraads
  • J. P. Nater
    • 1
  1. 1.Occupational Dermatology UnitState University HospitalRB GroningenThe Netherlands

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