Advertisement

Transformation in Spruce (Picea Species)

  • D. Ellis
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

The availability of a wide variety of regeneration systems makes Picea one of the best model species within the gymnosperms for genetic engineering. Tissue culture systems exist for the regeneration of whole plants from several Picea tissues as well as the regeneration of plants from protoplasts, embryogenic callus, and suspension cultures (Attree et al. 1991; Harry and Thorpe 1991). Genes have been transferred into and expressed in virtually all tissues capable of regeneration in Picea by a wide array of different gene transfer methods. Numerous Agrobacterium strains infectious to Picea spp. have been identified, and expression of the Agrobacterium T-DNA genes has been confirmed by opine production. Electroporation and direct DNA uptake with PEG have been used to introduce and express foreign genes in protoplasts from two Picea spp. Further, the use of particle acceleration has demonstrated that genes could be inserted and expressed in virtually every tissue thus far tested in Picea including megagametophytes, embryos, embryogenic callus, seedlings, and meristems. Finally, particle acceleration has been used to stably integrate foreign DNA into Picea glauca (white spruce) and transformed plants have been regenerated which contain and express the inserted genes. Clearly, the adaptability of various gene transfer methods from angiosperms to Picea have been the key to the success of gene transfer in this genus.

Keywords

Somatic Embryo Embryogenic Callus Zygotic Embryo Chloramphenicol Acetyl Transferase Transient Gene Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahuja MR (1988) Gene transfer in forest trees. In: Hanover JW, Keathley DE (eds) Genetic manipulation of woody plants. Plenum New York, pp. 25–41Google Scholar
  2. Attree SM, Dunstan DI, Fowke LC (1989) Initiation of embryogenic callus and suspension cultures, and improved embryo regeneration from protoplasts of white spruce (Picea glauca). Can J Bot 67:1709–1795CrossRefGoogle Scholar
  3. Attree SM, Dunstan DI, Fowke LC (1991) White spruce [Picea glauca (Moench) Voss] and black spruce [Picea mariana (Mill) B.S.P.]. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 16. Trees III. Springer, Berlin Heidelberg New York, pp 423–443Google Scholar
  4. Bekkaoui F, Pilon M, Laine E, Raju DSS, Crosby WL, Dunstan DI (1988) Transient gene expression in electroporated Picea glauca protoplasts. Plant Cell Rep. 7:481–484CrossRefGoogle Scholar
  5. Bekkaoui F, Datla RSS, Pilon M, Tautorus TE, Crosby WL, Dunstan DI (1990) The effects of promoter on transient expression in conifer cell lines. Theor Appl Genet 79:353–359CrossRefGoogle Scholar
  6. Callis J, Raasch JA, Vierstra RD (1990) Ubiquitin extension proteins of Arabidopsis thaliana. J Biol Chem 265 (21) 12486–12493PubMedGoogle Scholar
  7. Clapham DH, Ekberg I (1986) Induction of tumours by various strains of Agrobacterium tumefaciens on Abies nordmanniana and Picea abies. Scand J For Res 1:435–437CrossRefGoogle Scholar
  8. Clapham D, Ekberg I, Eriksson G Hood EE, Norell L. (1990) Within-population variation in susceptibility to Agrobacterium tumefaciens A281 in Picea abies (L.) Karst. Theor Appl Genet 79:654–656CrossRefGoogle Scholar
  9. De Cleene M, De Ley J (1976) The host range of crown gall. Bot Rev 42(4): 389–466CrossRefGoogle Scholar
  10. Diner AM, Karnosky DF (1987) Differential responses of two conifers to in vitro inoculation with Agrobacterium rhizogenes. Eur J For Pathol 17:211–216CrossRefGoogle Scholar
  11. Duchesne LC, Charest PJ (1991) Transient expression of the β-glucuronidase gene in embryogenic callus of Picea mariana following microprojection. Plant Cell Rep 10:19–194Google Scholar
  12. Ellis D, Roberts D, Sutton B, Lazaroff W, Webb D, Flinn B (1989a) Transformation of white spruce and other conifer species by Agrobacterium tumefaciens. Plant Cell Rep 8:16–20Google Scholar
  13. Ellis DD, Lazaroff WR, Roberts DR, Flinn BS, and Webb DT (1989b) The effect of antibiotics on elongation and callus and bud formation from embryogenic tissue of Picea glauca. Can J For Res 19:1343–1346Google Scholar
  14. Ellis DD, McCabe D, Russell D, Martinell B, McCown BH (1991) Expression of inducible angiosperm promoters in a gymnosperm, Picea glauca (white spruce). Plant Mol Biol 17:19–27Google Scholar
  15. Ellis DD, McCabe DE, Mclnnis S, Ramachandran R, Russell DR, Wallace KM, Martinell BJ, Roberts DR, Raffa KF, McCown BH (1993) Stable transformation of Picea glauca by particle acceleration. Bio/Tech 11(1): 84–89Google Scholar
  16. Harry IS, Thorpe TA. (1991) Engelmann spruce (Picea engelmannii Parry ex. Engelm). In:Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 16. Trees III. Springer, Berlin Heidelberg New York, pp. 408–422Google Scholar
  17. Hood EE, Clapham DH, Ekberg I, Johannson T (1990) T-DNA presence and opine production in tumors of Picea abies (L.) Karst induced by Agrobacterium tumefaciens A281. Plant Mol Biol 14:111–117Google Scholar
  18. Huang Y, Diner AM, Karnosky DF (1991) Agrobacterium rhizogenes-mediated genetic transformation and regeneration of a conifer: Larix decidua. In Vitro Cell Dev Biol 27P: 201–207Google Scholar
  19. Hutchison KW, Harvie PD, Singer PB, Brunner AF, Greenwood MS (1990) Nucleotide sequence of the small subunit of ribulose-1,5-bisphosphate carboxylase from the conifer Larix laricina. Plant Mol Biol 14:281–284Google Scholar
  20. Loopstra CA, Stomp A-M, Sederoff RR (1990) Agrobacterium-mediated DNA transfer in sugar pine. Plant Mol Biol 15:1–9Google Scholar
  21. Marcotte WR, Bay ley CC, Quatrano RS (1988) Regulation of a wheat promoter by abscisic acid in rice protoplasts. Nature 335:454–457Google Scholar
  22. McCown BH, McCabe DE, Rusell DR, Robinson DJ, Barton KA, Raffa KF (1991) Stable transformation of Populus and incorporation of pest resistance by electric discharge particle acceleration. Plant Cell Rep 9:590–594CrossRefGoogle Scholar
  23. Morris JW, Castle LA, Morris RO (1989) Efficacy of different Agrobacterium tumefaciens strains in transformation of pinaceous gymnosperms. Physiol Mol Plant Pathol 34:451–461Google Scholar
  24. Nagmani R, Becwar MR, Wann SR (1987) Single-cell origin and development of somatic embryos in Picea abies (L.) Karst. (Norway spruce) and P. glauca (Moench) Voss (white spruce). Plant Cell Rep 6:157–159Google Scholar
  25. Robertson D, Ackley R, Weissinger AK, Stomp A-M, Sederoff RR (1991) Stable transformation of Norway spruce embryogenic callus through microprojectile bombardment. In: Hallick, RB (ed) Third Int Congr Plant molecular biology, Tucson AZ, Program and Abstracts # 1036Google Scholar
  26. Schoffl F, Raschke E, Nagao RT (1984) The DNA sequence analysis of soybean heat-shock genes and identification of possible regulatory promoter elements. EMBO J 3:2491–2497Google Scholar
  27. Sellmer J (1991) Examination and manipulation of Populus cell competence for direct gene transfer. PhD Thesis, University of Wisconsin MadisonGoogle Scholar
  28. Serres R, Stang E, McCabe D, Rusell D, Mahr D, McCown B (1992) Gene transfer using electric discharge particle bombardment and recovery of transformed cranberry plants. J Am Soc Hortic Sci 117(1): 174–180Google Scholar
  29. Stomp A-M, Loopstra C, Sederoff R, Chilton S, Fillatti J, Dupper G, Tedeschi P, Kinlaw C (1988) Development of a DNA transfer system for pines. In: Hanover, JW. Keathley DE (eds) Genetic manipulation of woody plants. Plenum New York, pp 231–241Google Scholar
  30. Stomp AM, Loopstra C, Chilton WS, Sederoff RR, Moore LW (1990) Extended host range of Agrobacterium tumefaciens in genus Pinus. Plant Physiol 92:1226–1232PubMedCrossRefGoogle Scholar
  31. Tautorus TE, Bekkaoui F, Pilon M, Datla RSS, Crosby WL, Fowke LC, Dunstan DI (1989) Factors affecting transient gene expression in electroporated black spruce (Picea mariana) and jack pine (Pinus banksiana) protoplasts. Theor Appl Genet 78:531–536CrossRefGoogle Scholar
  32. Timko MP, Herdies L, de Almeida E, Cashmore AR, Leemans J, Krebbers E (1988) Genetic engineering of nuclear-encoded components of the photosynthetic apparatus in Arabidopsis. In: The impact of chemistry on biotechnology. ACS Symp Ser 362:279–295CrossRefGoogle Scholar
  33. von Arnold S, Eriksson T (1986) Norway spruce (Picea abies L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 1. Treess I. Springer, Berlin Heidelberg New York, pp 291–310Google Scholar
  34. Wilson SM, Thorpe TA, Moloney MM (1989) PEG-mediated expression of GUS and CAT genes in protoplasts from embryogenic suspension cultures of Picea glauca. Plant Cell Rep 7:704–707Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • D. Ellis
    • 1
  1. 1.Department of HorticultureUniversity of Wisconsin-MadisonMadisonUSA

Personalised recommendations