Transformation of Petunia and Corn Plants (Petunia hybrida and Zea mays) Using Agrobacterium tumefaciens and the Shoot Apex
Agrobacterium tumefaciens has evolved a reliable mechanism to transfer DNA into the genome of plant cells and is the most efficient gene vector available. However, the host range of the bacterium has been thought to be restricted to a narrow range of plant species (DeCleene 1985). This range is generally considered to include all dicotyledonous families; however, many dicot species are refractory to infection and members of some monocotyledonous families can be transformed. Furthermore, the apparent requirement for the regeneration of plants from callus or embryogenic callus imposed serious limits to the practical range of plants that could be transformed using A. tumefaciens.
KeywordsShoot Apex Agrobacterium Tumefaciens Corn Plant Petunia Hybrida Maize Streak Virus
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